Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians
Ning, Junhao1; Cao, Weian2; Lu, Xia1; Chen, Min1; Liu, Bo2; Wang, Chunde1,2
发表期刊COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
ISSN1096-4959
2021-10-01
卷号256页码:8
关键词Argopecten irradians irradiansFoxl2Expression analysisRNA interference
DOI10.1016/j.cbpb.2021.110638
通讯作者Wang, Chunde(chundewang2007@163.com)
英文摘要Transcription factor Foxl2 is an evolutionarily conserved gene playing pivotal roles in regulation of early ovarian differentiation and maintenance in animals. However, the Foxl2 gene has not been thoroughly studied in hermaphroditic scallops. In this study, we cloned and characterized a Foxl2 (designated as AiFoxl2) from the bay scallop Argopecten irradians irradians. The open reading frame of AiFoxl2 was 1122 bp encoding 373 amino acids residues and contained a conserved forkhead box domain. Quantitative real-time PCR showed that AiFoxl2 was mainly expressed in the ovary. Moreover, the highest expression of AiFoxl2 in the ovary was detected at proliferative stage and growing stage, while the lowest level was found at resting stage. During the embryonic and larval development, expression of AiFoxl2 was found first in fertilized eggs, increased significantly at the blastula stage, and reached peak value at the D-larvae stage. When AiFoxl2 was knocked down, testis development-related genes (Dmrt1, Sox7 and Sox9) were up-regulated significantly while the ovary development-related genes (Vg, HSD14, and GATA-1) were down-regulated manifestly. These findings suggested that AiFoxl2 was a femalerelated gene in A. i. irradians and may be involved in regulation of ovarian development and differentiation.
资助机构National Natural Science Foundation of China; Scientific and Technological Project of Yantai, Shandong Province; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]MOLECULAR-CLONING; GENE-EXPRESSION; DMRT1; DIFFERENTIATION; MAINTENANCE; TILAPIA; TRAITS; CELLS
研究领域[WOS]Biochemistry & Molecular Biology; Zoology
WOS记录号WOS:000684852500021
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29656
专题海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
中国科学院烟台海岸带研究所

通讯作者Wang, Chunde作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao 266109, Peoples R China

推荐引用方式
GB/T 7714Ning, Junhao,Cao, Weian,Lu, Xia,et al. Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians[J]. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,2021,256:8.
APANing, Junhao,Cao, Weian,Lu, Xia,Chen, Min,Liu, Bo,&Wang, Chunde.(2021).Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians.COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY,256,8.
MLANing, Junhao,et al."Identification and functional analysis of a sex-biased transcriptional factor Foxl2 in the bay scallop Argopecten irradians irradians".COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY 256(2021):8.


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