Gong, Fanwu1; Wei, Hua-xing2; Qi, Ji3; Ma, Huan1; Liu, Lianxin4; Weng, Jianping5; Zheng, Xucai1; Li, Qiangsheng1; Zhao, Dan1; Fang, Haopeng1; Liu, Liu1; He, Hongliang2; Ma, Cuichen1; Han, Jinglong6; Sun, Anyuan2; Wang, Baolong2; Jin, Tengchuan1; Li, Bowei3
; Li, Bofeng1发表期刊ACS SENSORS
ISSN2379-3694
2021-07-23
卷号6期号:7页码:2709-2719
关键词SARS-Cov-2COVID-19 diagnosispaper-based ELISAanti-RBD antibodyserum separationmicrofluidic devices
DOI10.1021/acssensors.1c00773
通讯作者Jin, Tengchuan(jint@ustc.edu.cn); Li, Bowei(bwli@yic.ac.cn); Li, Bofeng(libf@ustc.edu.cn)
英文摘要The spread of Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome-coronavirus 2 (SARS-CoV-2), resulting in a global pandemic with around four million deaths. Although there are a variety of nucleic acid-based tests for detecting SARS-CoV-2, these methods have a relatively high cost and require expensive supporting equipment. To overcome these limitations and improve the efficiency of SARS-CoV-2 diagnosis, we developed a microfluidic platform that collected serum by a pulling-force spinning top and paper-based microfluidic enzyme-linked immunosorbent assay (ELISA) for quantitative IgA/IgM/IgG measurements in an instrument-free way. We further validated the paper-based microfluidic ELISA analysis of SARS-CoV-2 receptor-binding domain (RBD)-specific IgA/IgM/IgG antibodies from human blood samples as a good measurement with higher sensitivity compared with traditional IgM/IgG detection (99.7% vs 95.6%) for early illness onset patients. In conclusion, we provide an alternative solution for the diagnosis of SARS-CoV-2 in a portable manner by this smart integration of pulling-force spinning top and paper-based microfluidic immunoassay.
资助机构National Natural Science Foundation of China; COVID-19 special task grant from Chinese Academy of Science Clinical Research Hospital (Hefei); Shandong Provincial Natural Science Foundation Key Project; China Postdoctoral Science Foundation
收录类别SCI
语种英语
关键词[WOS]ANTIBODIES; CHIP
研究领域[WOS]Chemistry; Science & Technology - Other Topics
WOS记录号WOS:000677583700023
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29598
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Jin, Tengchuan; Li, Bowei; Li, Bofeng作者单位1.Univ Sci & Technol China, Affiliated Hosp USTC 1, Dept Med Oncol, Div Life Sci & Med, Hefei 230001, Peoples R China
2.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Lab Med, Hefei 230001, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
4.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Hepatobiliary Surg, Hefei 230021, Peoples R China
5.Univ Sci & Technol China, Div Life Sci & Med, Affiliated Hosp USTC 1, Dept Endocrinol & Metab, Hefei 230027, Peoples R China
6.Yantai Univ, Sch Environm & Mat Engn, Yantai 264005, Peoples R China
推荐引用方式
GB/T 7714Gong, Fanwu,Wei, Hua-xing,Qi, Ji,et al. Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis[J]. ACS SENSORS,2021,6(7):2709-2719.
APAGong, Fanwu.,Wei, Hua-xing.,Qi, Ji.,Ma, Huan.,Liu, Lianxin.,...&Li, Bofeng.(2021).Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis.ACS SENSORS,6(7),2709-2719.
MLAGong, Fanwu,et al."Pulling-Force Spinning Top for Serum Separation Combined with Paper-Based Microfluidic Devices in COVID-19 ELISA Diagnosis".ACS SENSORS 6.7(2021):2709-2719.
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