Liu, Ping1,2; Fu, Longwen2,3
; Song, Zhihua4; Man, Mingsan2; Yuan, Huamao3,5; Zheng, Xiaoli1; Kang, Qi1; Shen, Dazhong1; Song, Jinming3; Li, Bowei2,3; Chen, Lingxin2,3发表期刊ANALYST
ISSN0003-2654
2021-07-29
页码9
DOI10.1039/d1an00927c
通讯作者Shen, Dazhong(dzshen@sdnu.edu.cn); Li, Bowei(bwli@yic.ac.cn); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要In this article, we present a novel nitrocellulose-based microfluidic chip with 3-dimensional (3D) printing technology to study the effect of oxygen gradient on cells. Compared with conventional polydimethylsiloxane (PDMS) chips of oxygen gradient for cell cultures that can only rely on fluorescence microscope analysis, this hybrid nitrocellulose-based microfluidic platform can provide a variety of analysis methods for cells, including flow cytometry, western blot and RT-PCR, because the nitrocellulose-based chips with cells can be taken out from the growth chambers of 3D printed microfluidic chip and then used for cell collection or lysis. These advantages allow researchers to acquire more information and data on the basic biochemical and physiological processes of cell life. The effect of oxygen gradient on the zebrafish cells (ZF4) was used as a model to show the performance and application of our platform. Hypoxia caused the increase of intercellular reactive oxygen species (ROS) and accumulation of hypoxia-inducible factor 1 alpha (HIF-1 alpha). Hypoxia stimulated the transcription of hypoxia-responsive genes vascular endothelial growth factor (VEGF) and induced cell cycle arrest of ZF4 cells. The established platform is able to obtain more information from cells in response to different oxygen concentration, which has potential for analyzing the cells under a variety of pathological conditions.
资助机构National Natural Science Foundation of China; National Key Research and Development Program of China; Key Deployment Project of Centre for Ocean Mega-Research of Science, Chinese Academy of Sciences; Open Fund of CAS Key Laboratory of Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]GROWTH-FACTOR VEGF; FLUORESCENCE DETECTION; CANCER-CELLS; HYPOXIA; GENERATION; ZEBRAFISH; CULTURE; DEVICE; CHIP; ANGIOGENESIS
研究领域[WOS]Chemistry
WOS记录号WOS:000678901200001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29558
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
通讯作者Shen, Dazhong; Li, Bowei; Chen, Lingxin作者单位1.Shandong Normal Univ, Minist Educ, Key Lab Mol & Nano Probes, Coll Chem,Chem Engn & Mat Sci, Jinan 250014, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
4.Yantai Univ, Minist Educ, Sch Pharm, Key Lab Mol Pharmacol & Drug Evaluat, Yantai 264005, Peoples R China
5.Chinese Acad Sci, Inst Oceanol, Key Lab Marine Ecol & Environm Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Liu, Ping,Fu, Longwen,Song, Zhihua,et al. Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells[J]. ANALYST,2021:9.
APALiu, Ping.,Fu, Longwen.,Song, Zhihua.,Man, Mingsan.,Yuan, Huamao.,...&Chen, Lingxin.(2021).Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells.ANALYST,9.
MLALiu, Ping,et al."Three dimensionally printed nitrocellulose-based microfluidic platform for investigating the effect of oxygen gradient on cells".ANALYST (2021):9.
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