Lu, Pengpeng1,2; Zhang, Caiyun1; Fu, Lili1,2; Wei, Yinghui2,3; Huang, Yan1,2; Wang, Xiaoyan1,2; Lv, Changjun3; Chen, Lingxin1,2,4
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2021-07-27
卷号93期号:29页码:10378-10387
DOI10.1021/acs.analchem.1c02386
通讯作者Huang, Yan(yhuang@yic.ac.cn); Wang, Xiaoyan(wangxy@yic.ac.cn); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Pantetheinase (also known as Vanin-1) is highly expressed in the liver, kidneys, and intestine and is closely associated with a number of diseases. Vanin-1 can hydrolyze pantetheine to pantothenic acid (vitamin B5) and cysteamine and participate in the synthesis of glutathione (GSH). GSH is highly expressed in tumor cells and plays a major role in the resistance of tumor cells to cisplatin. Therefore, we urgently need a method to monitor the activity level of Vanin-1 in tumor cells and tissues and elucidate the relationship between the role of Vanin-1 in GSH synthesis and tumor resistance. Herein, we report a Cy-Pa fluorescent probe for imaging Vanin-1 in cells and in vivo that can qualitatively and quantitatively detect the fluctuation of Vanin-1 concentrations in HepG2 and HepG2/DDP cells or tumor tissues of tumor-bearing mice. This probe shows excellent potential in in situ real-time monitoring of endogenous Vanin-1. Moreover, we proved that Vanin-1 can inhibit GSH synthesis using the probe. When the Vanin-1 inhibitor RR6 was used in combination with cisplatin, HepG2 and HepG2/DDP cells showed increased resistance to cisplatin, while the therapeutic efficiency of cisplatin was reduced in HepG2 and HepG2/DDP xenografts. In this study, Vanin-1 was shown to play an important role in the treatment of cancer, and the study of Vanin-1 may provide an idea for the treatment of cancer in the future.
资助机构National Nature Science Foundation of China; Science and Technology Innovation Development Plan of Yantai of China; Research Initiation Fund of Binzhou Medical University; Taishan Scholar Project Special Fund; Shandong Peninsula Engineering Research Center of Comprehensive Brine Utilization
收录类别SCI
语种英语
关键词[WOS]DRUG-RESISTANCE; URINARY VANIN-1; CANCER; GLUTATHIONE; CISPLATIN; PANTETHEINASE; ASSAY; CYSTEAMINE; METABOLISM; PATHWAY
研究领域[WOS]Chemistry
WOS记录号WOS:000679366000052
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29552
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Huang, Yan; Wang, Xiaoyan; Chen, Lingxin作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
3.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China
4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式
GB/T 7714Lu, Pengpeng,Zhang, Caiyun,Fu, Lili,et al. Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy[J]. ANALYTICAL CHEMISTRY,2021,93(29):10378-10387.
APALu, Pengpeng.,Zhang, Caiyun.,Fu, Lili.,Wei, Yinghui.,Huang, Yan.,...&Chen, Lingxin.(2021).Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy.ANALYTICAL CHEMISTRY,93(29),10378-10387.
MLALu, Pengpeng,et al."Near-Infrared Fluorescent Probe for Imaging and Evaluating the Role of Vanin-1 in Chemotherapy".ANALYTICAL CHEMISTRY 93.29(2021):10378-10387.
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