Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene
Yuan, Xiutang1,2; Zhao, Huan3; Wang, Yinan2,3; Wang, Lili2; Li, Dongmei2; Zhang, Anguo2; Yang, Xiaolong2; Ma, Xindong2; Yang, Dazuo3; Zhou, Yibing3
发表期刊MARINE POLLUTION BULLETIN
ISSN0025-326X
2021-08-01
卷号169页码:6
关键词PolychaetePAH biotransformationGSTLiaohe Estuary
DOI10.1016/j.marpolbul.2021.112552
通讯作者Yuan, Xiutang(xtyuan@yic.ac.cn)
英文摘要Polychaete worms can eliminate polycyclic aromatic hydrocarbons (PAHs) in environments through a mechanism that increases their water solubility. This detoxification starts with cytochrome P450 enzymes (CYPs) and then with glutathione S-transferases (GSTs). Here, a novel GST gene was identified and characterized from the widespread polychaete Perinereis aibuhitensis. The full-length cDNA of GST is 1544 bp and encodes 256 amino acids, belonging to the omega class. Gene expression patterns in P. aibuhitensis showed that its transcriptional level was positively correlated with the concentration of benzo[alpha]pyrene (0.5, 2, 4, and 8 mu g/L) exposure but was negatively correlated with a PAH benzene ring after it was exposed to the same mass concentrations of fluoranthene (3.2 mu g/L), phenanthrene (2.9 mu g/L), and benzo[alpha]pyrene (4.0 mu g/L) during the 14-day experimentation. These findings indicate that omega GST may play an important role in the phase II detoxification of PAHs in polychaete worms, and the persistence and bioavailability of PAHs may depend on benzene rings.
资助机构National Natural Science Foundation of China; National Marine Public Welfare Research Project
收录类别SCI
语种英语
关键词[WOS]POLYCYCLIC AROMATIC-HYDROCARBONS; MOLECULAR-CLONING; PAHS; METABOLITES; SEDIMENT; ESTUARY; NUNTIA
研究领域[WOS]Environmental Sciences & Ecology; Marine & Freshwater Biology
WOS记录号WOS:000679313100012
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29546
专题海岸带生物学与生物资源利用重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室

通讯作者Yuan, Xiutang作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Minist Ecol & Environm, Natl Marine Environm Monitoring Ctr, Dalian 116023, Peoples R China
3.Dalian Ocean Univ, Dalian 116023, Peoples R China

推荐引用方式
GB/T 7714Yuan, Xiutang,Zhao, Huan,Wang, Yinan,et al. Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene[J]. MARINE POLLUTION BULLETIN,2021,169:6.
APAYuan, Xiutang.,Zhao, Huan.,Wang, Yinan.,Wang, Lili.,Li, Dongmei.,...&Zhou, Yibing.(2021).Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene.MARINE POLLUTION BULLETIN,169,6.
MLAYuan, Xiutang,et al."Expression profile of a novel glutathione S-transferase gene in the marine polychaete Perinereis aibuhitensis in short-term responses to phenanthrene, fluoranthene, and benzo[alpha]pyrene".MARINE POLLUTION BULLETIN 169(2021):6.


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