Miao, Qin1,2,3; Mi, Yingqi1,2,3; Cui, Jingmin1,2,3; Zhang, Jingjing1,2; Tan, Wenqiang1,2
; Li, Qing1,2; Guo, Zhanyong1,2,3发表期刊INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
ISSN0141-8130
2021-07-01
卷号182页码:1537-1542
关键词Determination of chitosan contentChitosan-Schiff baseHPLC
DOI10.1016/j.ijbiomac.2021.05.121
通讯作者Guo, Zhanyong(zhanyongguo@hotmail.com)
英文摘要Tremendous awareness of determination of chitosan content accurately is increasing, due to it has great significance to the quality control of chitosan. In this article, two kinds of chitosan-Schiff base derivatives (BCSB and PCSB) were synthesized by the different average degrees of deacetylation (DD) of chitosan with benzaldehyde or propanal, respectively. The total mass of Schiff base derivative product was dried and obtained without washing and loss. Then, a certain amount of the prepared Schiff base compound was taken to hydrolyze into glucosamine hydrochloride (GAH) in strong hydrochloric acidic environment, whose concentration was quantified by HPLC, and the mass of GAH contained in hydrolysis solution could be calculated. Subsequently, the total quality of GAH obtained by hydrolysis of all of the Schiff base product was calculated and obtained, and then the theoretical mass of chitosan could be deduced and calculated by further converse calculation. Finally, the chitosan content was obtained by combining the sample mass used in Schiff base reaction and the theoretical mass of chitosan. This method was accurate and convenient, providing a preeminent idea and method for the determination of chitosan content. (c) 2021 Elsevier B.V. All rights reserved.
资助机构National Key Research and Development Program of China; Natural Science Foundation of Shandong Province of China
收录类别SCI
语种英语
关键词[WOS]LIQUID-CHROMATOGRAPHIC DETERMINATION; ACID-HYDROLYSIS; ANTIMICROBIAL ACTIVITY; GLUCOSAMINE; CHITIN; DEACETYLATION; STABILITY; SAMPLES; CRAB
研究领域[WOS]Biochemistry & Molecular Biology; Chemistry; Polymer Science
WOS记录号WOS:000669494700003
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29531
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Guo, Zhanyong作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Shandong, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Miao, Qin,Mi, Yingqi,Cui, Jingmin,et al. Determination of chitosan content with Schiff base method and HPLC[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2021,182:1537-1542.
APAMiao, Qin.,Mi, Yingqi.,Cui, Jingmin.,Zhang, Jingjing.,Tan, Wenqiang.,...&Guo, Zhanyong.(2021).Determination of chitosan content with Schiff base method and HPLC.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,182,1537-1542.
MLAMiao, Qin,et al."Determination of chitosan content with Schiff base method and HPLC".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 182(2021):1537-1542.
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