Wang, Xiaokun1; Shang, Hezhen2; Ma, Cuiping1; Chen, Lingxin3,4,5
发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2021-06-22
卷号93期号:24页码:8493-8500
DOI10.1021/acs.analchem.1c00796
通讯作者Ma, Cuiping(mcp169@163.com); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Exosomes are emerging as one of the most promising biomarkers for early disease diagnosis and prognosis. The significant challenges facing the available methods include improving the detection specificity and sensitivity in complex biological samples. Herein, a fluorescence assay was established based on a combination of immunomagnetic separation and a two-step signal amplification strategy for direct isolation and subsequent detection of exosomes. First, immunomagnetic beads capture and enrich the exosomes via antibody-antigen reactions. Second, bivalent cholesterol (BC) anchors spontaneously insert into the lipid bilayer of bead-captured exosomes, forming a "one to many" amplification effect. The simultaneous recognition of the surface protein and the lipid bilayer structure of the exosome significantly eliminates the interference risk from free proteins. The detection of exosomes converts to the detection of BC-anchors. Finally, the sticky end of the BC-anchor acts as the initiator to trigger the enzyme-free DNA circuits for secondary signal amplification. Under the optimal conditions, highly sensitive and selective detection of exosomes was achieved ranging from 5.5 x 10(3) to 1.1 x 10(7) particles/mu L with a limit of detection of 1.29 x 10(3) particles/mu L. Moreover, this method allows the isolation and quantitative analysis of exosomes in several biological fluids with satisfactory recovery rates (92.25-106.8%). Thus, this approach provides a sensitive, anti-interference platform for isolating and detecting exosomes.
资助机构National Nature Science Foundation of China; Taishan Scholar Project Special Funding; Key Project of Natural Science Foundation of Shandong Province
收录类别SCI
语种英语
关键词[WOS]EXTRACELLULAR VESICLES; CANCER EXOSOMES; CELL; SECRETION; PROTEINS; BIOGENESIS; PROGRESS
研究领域[WOS]Chemistry
WOS记录号WOS:000665642500016
引用统计被引频次：6[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29465
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Ma, Cuiping; Chen, Lingxin作者单位1.Qingdao Univ Sci & Technol, Shandong Prov Key Lab Biochem Engn, Coll Marine Sci & Biol Engn, Qingdao 266042, Peoples R China
2.Qingdao Chengyang Dist Peoples Hosp, Dept Hepatobiliary Surg, Qingdao 266109, Peoples R China
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
4.Qufu Normal Univ, Key Lab Life Organ Anal, Key Lab Pharmaceut Intermediates & Anal Nat Med, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
5.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Wang, Xiaokun,Shang, Hezhen,Ma, Cuiping,et al. A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification[J]. ANALYTICAL CHEMISTRY,2021,93(24):8493-8500.
APAWang, Xiaokun,Shang, Hezhen,Ma, Cuiping,&Chen, Lingxin.(2021).A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification.ANALYTICAL CHEMISTRY,93(24),8493-8500.
MLAWang, Xiaokun,et al."A Fluorescence Assay for Exosome Detection Based on Bivalent Cholesterol Anchor Triggered Target Conversion and Enzyme-Free Signal Amplification".ANALYTICAL CHEMISTRY 93.24(2021):8493-8500.
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