Zhang, Han1,2,5; Mou, Junsong1,2,5; Ding, Jiawang1,2,3,4
; Qin, Wei1,2,3,4发表期刊ELECTROCHIMICA ACTA
ISSN0013-4686
2021-07-10
卷号384页码:8
关键词PotentiometricPeptideMagnetic fieldBacteriaOuter-membrane protease T
DOI10.1016/j.electacta.2021.138408
通讯作者Ding, Jiawang(jwding@yic.ac.cn)
英文摘要Rapid, sensitive and reliable Escherichia coli (E. coli) detection and identification are critically important to protect public health. Here, we describe a magneto-controlled potentiometric assay for specific detection of E. coli cells by making use of the E. coli outer-membrane protease T (OmpT). OmpT is an endopepti-dase that specifically cleaves peptide at dibasic sites. A rationally designed peptide serving as both OmpT substrate and potentiometric signal reporter was immobilized on magnetic beads. The rapid accumu-lation and extraction of peptide-functionalized magnetic beads on a polymeric membrane doped with an ion exchanger can be achieved using a magnetic force. The magnetic-field-assisted extraction of the peptide into the polymeric membrane ion-sensitive sensor, as confirmed by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy, can lead to a rapid, stable and reproducible potential change. OmpT is capable of cleaving the positively charged peptide on the magnetic beads, thus resulting in charge density change. The change in charge density and subsequently the potential change can be readily detected and used for quantification of E. coli at levels down to 5.0 x 10(-3) CFU mL(-1). This work provides a versatile, rapid and reliable potentiometric method for E. coli detection. (C) 2021 Elsevier Ltd. All rights reserved.
资助机构National Natural Science Foundation of China; Instrument Developing Project of the Chinese Academy of Sciences; Taishan Scholar Program of Shandong Province; Key Research and Development Program of Yantai
收录类别SCI
语种英语
关键词[WOS]ESCHERICHIA-COLI; SUBSTRATE-SPECIFICITY; WATER; PROTAMINE; PROTEINS; BEADS
研究领域[WOS]Electrochemistry
WOS记录号WOS:000651772000003
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29319
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
通讯作者Ding, Jiawang作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Shandong, Peoples R China
2.YICCAS, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Shandong, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Zhang, Han,Mou, Junsong,Ding, Jiawang,et al. Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T[J]. ELECTROCHIMICA ACTA,2021,384:8.
APAZhang, Han,Mou, Junsong,Ding, Jiawang,&Qin, Wei.(2021).Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T.ELECTROCHIMICA ACTA,384,8.
MLAZhang, Han,et al."Magneto-controlled potentiometric assay for E. coli based on cleavage of peptide by outer-membrane protease T".ELECTROCHIMICA ACTA 384(2021):8.
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