Yang, Guilan1,2; Qin, Song1,3
; Li, Wenjun1,3发表期刊JOURNAL OF FOOD SCIENCE
ISSN0022-1147
2021-05-30
页码11
关键词Alaska pollack skins protein hydrolysateangiotensin I-converting enzyme (ACE)-inhibitory peptidegel filtration chromatographymolecular dockingultrafiltration
DOI10.1111/1750-3841.15754
通讯作者Li, Wenjun(wjli@yic.ac.cn)
英文摘要Hydrolysates containing angiotensin I-converting enzyme (ACE)-inhibitory peptide were prepared from protein of Alaska pollack skins using alcalase and trypsin. The protein hydrolysate was separated by ultrafiltration, Sephadex G-25 gel filtration chromatography and reversed phase high-performance liquid chromatography (HPLC), from which a novel purified peptide was obtained. Both random coil structure and beta-sheet in the purified peptide were revealed in Fourier transform infrared spectrum. The amino sequence of the purified peptide was identified as GPLGVP, VLYPVK, VFLENVLR, and FEEF by HPLC-Q-TOF-MS (HPLC-quadrupole time-of-flight mass spectrometry). The peptide GPLGVP whose molecular weight was 538.31 Da showed the highest ACE inhibitory activity (IC50 = 105.8 mu M). The purified peptide featured a noncompetitive inhibition kinetic mechanism was shown in the Lineweaver-Burk plots and was susceptible to enzymes as indicated in the studies on stability of gastrointestinal proteases. Moreover, the peptide GPLGVP can combine ACE catalytic pocket through hydrogen bonds and other forces with high binding power as disclosed in molecular docking simulation, which provides the inhibitory effect of GPLGVP on ACE.
资助机构National Key Research and Development Program of China; Marine Economy Innovation Development Demonstration Project
收录类别SCI
语种英语
关键词[WOS]PROTEIN HYDROLYSATE; ACE; STABILITY; KINETICS; MUSCLE; VENOM
研究领域[WOS]Food Science & Technology
WOS记录号WOS:000656102700001
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29298
专题海岸带生物学与生物资源利用重点实验室
中国科学院烟台海岸带研究所
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Li, Wenjun作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
2.Binzhou Med Univ, Sch Pharm, Yantai, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Yang, Guilan,Qin, Song,Li, Wenjun. Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins[J]. JOURNAL OF FOOD SCIENCE,2021:11.
APAYang, Guilan,Qin, Song,&Li, Wenjun.(2021).Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins.JOURNAL OF FOOD SCIENCE,11.
MLAYang, Guilan,et al."Purification and characterization of a novel angiotensin I-converting enzyme-inhibitory peptide derived from Alaska pollack skins".JOURNAL OF FOOD SCIENCE (2021):11.
PDF全文下载地址:
点我下载PDF
