Wang, Xiaokun1; Wang, Xiudan1; Shi, Chao3; Ma, Cuiping1; Chen, Lingxin2,4
发表期刊TALANTA
ISSN0039-9140
2020-08-15
卷号216页码:7
关键词Lateral flow biosensorIsothermal amplificationLow resource settingNucleic acid testing
DOI10.1016/j.talanta.2020.120978
通讯作者Ma, Cuiping(mcp169@163.com); Chen, Lingxin(lxchen@yic.ac.cn)
英文摘要Amplification reactions coupled with electrophoresis or real-time fluorescence system have been successfully used for the analysis of nucleic acid. However, complex procedures or expensive instruments greatly limit their application in low-resource settings. To address these shortcomings, we fabricated a universal and simple detection platform by integrating strand exchange amplification (SEA) with lateral flow assay (LFA) strip. SEA is a simple isothermal amplification reaction, only requires a pair of primers and one DNA polymerase, above all, its short amplicons are easy to migrate on the strip. LFA strip was proved to be stable for months without large signal deviations and result could be easy to read by naked eyes, which makes it an appropriate option for field-based analysis. Our proposed SEA-LFA strip could reliably detect as few as 0.05 nM pork DNA and 0.07 nM duck DNA by the naked eye, its analytical performance is comparable with laboratory-based real-time fluorescence test. Furthermore, this proof-of-concept method could be applied to detect a wide variety of nucleic acid by focusing on primer design rather than on the development of a wholly new analysis platform. We believe that this simple visualization system has great potential as a preliminary test tool in resource-limited areas.
资助机构National Natural Science Foundation of China; Key Deployment Project of Centre for Ocean MegaResearch of Science, Chinese Academy of Science; Taishan Scholar Project Special Funding
收录类别SCI
语种英语
关键词[WOS]ISOTHERMAL AMPLIFICATION; GOLD NANOPARTICLES; EBOLA-VIRUS; DIAGNOSTICS; OLIGONUCLEOTIDE; IDENTIFICATION; DENATURATION; ADULTERATION; IMMUNOASSAY; SIZE
研究领域[WOS]Chemistry
WOS记录号WOS:000536207600039
引用统计被引频次：9[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/29081
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Ma, Cuiping; Chen, Lingxin作者单位1.Qingdao Univ Sci & Technol, Coll Marine Sci & Biol Engn, Shandong Prov Key Lab Biochem Engn, Qingdao 266042, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc, Yantai 264003, Peoples R China
3.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China
4.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Peoples R China
推荐引用方式
GB/T 7714Wang, Xiaokun,Wang, Xiudan,Shi, Chao,et al. Highly sensitive visual detection of nucleic acid based on a universal strand exchange amplification coupled with lateral flow assay strip[J]. TALANTA,2020,216:7.
APAWang, Xiaokun,Wang, Xiudan,Shi, Chao,Ma, Cuiping,&Chen, Lingxin.(2020).Highly sensitive visual detection of nucleic acid based on a universal strand exchange amplification coupled with lateral flow assay strip.TALANTA,216,7.
MLAWang, Xiaokun,et al."Highly sensitive visual detection of nucleic acid based on a universal strand exchange amplification coupled with lateral flow assay strip".TALANTA 216(2020):7.
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