Zhang, Yi1; Liu, Xiao1; Wang, Lingling1; Yang, Hanjie1; Zhang, Xiaoxiao1; Zhu, Chenglong1; Wang, Wenlong1; Yan, Lijing2; Li, Bowei3
发表期刊SCIENTIFIC REPORTS
ISSN2045-2322
2020-06-15
卷号10期号:1页码:9
DOI10.1038/s41598-020-66456-1
通讯作者Zhang, Yi(zhangyijnu@jiangnan.edu.cn)
英文摘要Lateral flow assay (LFA) is one of the most prevalent commercially available techniques for point-of-care tests due to its simplicity, celerity, low cost and robust operation. However, conventional colorimetric LFAs have inferior limits of detection (LODs) compared to sophisticated laboratory-based assays. Here, we report a simple strategy of test-zone pre-enrichment to improve the LOD of LFA by loading samples before the conjugate pad assembly. The developed method enables visual LODs of miR-210 mimic and human chorionic gonadotropin protein, to be improved by 10-100 fold compared with a conventional LFA setup without introducing any additional instrument and reagent except for phosphate running buffer, while no obvious difference occurred for Aflatoxin B1 (AFB1). It takes about 6-8min to enrich every 50 mu L of sample diluted with phosphate running buffer, therefore we can get visual results within 20min. We identified a parameter by modeling the entire process, the concentration of probe-analyte conjugate at test zone when signaling unit being loaded, to be important for the improvement of visual limit of detection. In addition, the test-zone pre-enrichment did not impair the selectivity when miR-210 mimic was adopted as target. Integrated with other optimization, amplification and modification of LFAs, the developed test-zone pre-enrichment method can be applied to further improve LOD of LFAs.
资助机构National Natural Science Foundation of China; Postgraduate Research & Practice Innovation Program of Jiangsu Provence
收录类别SCI
语种英语
关键词[WOS]RECOMBINASE POLYMERASE AMPLIFICATION; ULTRASENSITIVE DETECTION; VISUAL DETECTION; AFLATOXIN B-1; SINGLE-STEP; SENSITIVITY; IMMUNOASSAYS; SIGNAL
研究领域[WOS]Science & Technology - Other Topics
WOS记录号WOS:000544936100006
引用统计被引频次：9[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28635
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
通讯作者Zhang, Yi作者单位1.Jiangnan Univ, State Key Lab Food Sci & Technol,Sch Food Sci & T, Int Joint Lab Food Safety,Inst Analyt Food Safety, Collaborat Innovat Ctr Food Safety & Qual Control, Wuxi 214122, Jiangsu, Peoples R China
2.Jiangnan Univ Hosp, Wuxi 214122, Jiangsu, Peoples R China
3.Chinese Acad Sci, Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Zhang, Yi,Liu, Xiao,Wang, Lingling,et al. Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment[J]. SCIENTIFIC REPORTS,2020,10(1):9.
APAZhang, Yi.,Liu, Xiao.,Wang, Lingling.,Yang, Hanjie.,Zhang, Xiaoxiao.,...&Li, Bowei.(2020).Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment.SCIENTIFIC REPORTS,10(1),9.
MLAZhang, Yi,et al."Improvement in Detection Limit for Lateral Flow Assay of Biomacromolecules by Test-Zone Pre-enrichment".SCIENTIFIC REPORTS 10.1(2020):9.
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