Lv, Yingchun1,2
; Zhang, Yang2,3; Song, Jiawei2,3; Xu, Gang1,2发表期刊DESALINATION AND WATER TREATMENT
ISSN1944-3994
2020-02-01
卷号177页码:131-138
关键词Alkaline phosphataseKinetic characteristicsHeavy metalSwan Lake
DOI10.5004/dwt.2020.24924
通讯作者Xu, Gang(gxu@yic.ac.cn)
英文摘要Phosphatase-catalyzed hydrolysis of organic phosphorus is an important pathway for providing bioavailable phosphorus to aquatic organisms. In this paper, a eutrophic water body was used to investigate how phosphorus and heavy metal ions affect alkaline phosphatase activity (APA) and its kinetic properties. The results show that phosphate (PO43-), hexametaphosphate ((PO3)(6)(6-)), pyrophosphate (P2O74-), and beta-glycerophosphate (C3H4O6P2-) inhibit APA and the inhibition effects increased with increasing concentration at the range of 0.01-1 mmol L-1. Addition of heavy metal ions, that is, Co2+, Pb2+, and Cr6+ at concentrations of 1 mmol L-1, increased the APA 3.1-fold, 2.3-fold, and 2.7-fold, respectively. The Cu2+, Zn2+, and Ni2+ exhibited an inhibitory effect on APA in water. For 1 mmol L-1 Cu2+, the APA deceased by 37.7% relative to the control. As the concentrations of Cu2+, PO43-, (PO3)(6)(6-), and P2O74- increased, the V-max of the alkaline phosphatase (AP) remained stable and K-m increased, indicating that these ions are competitive inhibitors of AP. In addition, V-max/K-m decreased with increasing concentrations of Cu2+, PO43-, (PO3)(6)(6-), P2O74-, and C3H4O6P2-, demonstrating the decreased catalytic efficiency of the AP. In summary, phosphorus and heavy metal significantly affect conversion of organic phosphorus into bioavailable inorganic phosphorous by changing the APA in eutrophic water bodies.
资助机构National Natural Science Foundation of China; Key Deployment Project of the Chinese Academy of Sciences; Science and Technology Service Network Initiative
收录类别SCI
语种英语
关键词[WOS]DISSOLVED ORGANIC PHOSPHORUS; ENZYMES; IMPACT
研究领域[WOS]Engineering; Water Resources
WOS记录号WOS:000544993600013
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28632
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
中科院海岸带环境过程与生态修复重点实验室
通讯作者Xu, Gang作者单位1.Qufu Normal Univ, Coll Geog & Tourism, Rizhao 276826, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, 17 Chunhui Rd, Yantai 264003, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Lv, Yingchun,Zhang, Yang,Song, Jiawei,et al. Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals[J]. DESALINATION AND WATER TREATMENT,2020,177:131-138.
APALv, Yingchun,Zhang, Yang,Song, Jiawei,&Xu, Gang.(2020).Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals.DESALINATION AND WATER TREATMENT,177,131-138.
MLALv, Yingchun,et al."Regulation of the activity and kinetic properties of alkaline phosphatase in eutrophic water bodies by phosphorus and heavy metals".DESALINATION AND WATER TREATMENT 177(2020):131-138.
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