Lin, Jiliang1; Ning, Junhao2; Lu, Xia2
; Chen, Min2; Cao, Weian1; Wang, Chunde1,2发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2020-11-01
卷号106页码:365-373
关键词Scapharca subcrenataTranscriptomeMaternal immunityC-type lectinExpression analysis
DOI10.1016/j.fsi.2020.08.016
通讯作者Wang, Chunde(chundewang2007@163.com)
英文摘要Little information is available on innate immune defense mechanisms of Scapharca subcrenata. C-type lectins (CTLs) are not only pattern recognition proteins that can bind pathogen-associated molecular patterns, but also crucial maternally-derived immune factors in mollusc egg. In this study, the comparative transcriptome analysis of Vibrio parahaemolyticus-infected and untreated hepatopancreas were performed to identify the key genes involved in maternal transfer of immunity. A total of 3514 and 9327 differentially expressed genes (DEGs) were identified at 6 and 48 h post challenge compared to control groups. Gene Ontology and Cluster of Orthologous Groups analysis showed that most DEGs were classified under regulation of signal transduction, regulation of the metabolic process of carbohydrates and secondary metabolites, while the processes of posttranscriptional modification and protein translation were inhibited manifestly. The DEGs were most enriched in pathways related to lysosome, phagosome and EMC-receptor interaction. Among the DEGs, 191 maternal immune-related genes that could provide developing embryos a better protection against pathogen infection were identified according to previous studies. Additionally, five CTLs (designated as SsCTL1-5) identified from the DEGs were cloned, and their expression patterns in different tissues and post immune stimulation were analyzed. These findings would be beneficial for understanding the innate immune defense mechanisms of S. subcrenata.
资助机构National Key R&D Program of China; National Natural Science Foundation of China; Scientific and Technological Project of Yantai, Shandong Province, China; Key Deployment Projects of Center for Ocean Mega-Science, Chinese Academy of Sciences, China
收录类别SCI
语种英语
关键词[WOS]SCALLOP ARGOPECTEN-IRRADIANS; MATERNAL TRANSFER; CHLAMYS-FARRERI; FUNCTIONAL-CHARACTERIZATION; BINDING; EGG; IDENTIFICATION; RECOGNITION; COMPLEMENT; COMPONENTS
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000579494400036
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/28459
专题海岸带生物学与生物资源利用重点实验室
中国科学院烟台海岸带研究所
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
通讯作者Wang, Chunde作者单位1.Qingdao Agr Univ, Qingdao 266109, Shandong, Peoples R China
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Lin, Jiliang,Ning, Junhao,Lu, Xia,et al. Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata[J]. FISH & SHELLFISH IMMUNOLOGY,2020,106:365-373.
APALin, Jiliang,Ning, Junhao,Lu, Xia,Chen, Min,Cao, Weian,&Wang, Chunde.(2020).Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata.FISH & SHELLFISH IMMUNOLOGY,106,365-373.
MLALin, Jiliang,et al."Transcriptomic analysis and expression of C-type lectins in response to Vibrio parahaemolyticus challenge in Scapharca subcrenata".FISH & SHELLFISH IMMUNOLOGY 106(2020):365-373.
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