Cui, Yulin1,2
; Wang, Kang3; Xu, Wenxin4; Wang, Yinchu1,2; Gao, Zhengquan3; Cui, Hongli4; Meng, Chunxiao3; Qin, Song1,2发表期刊JOURNAL OF PHYCOLOGY
ISSN0022-3646
2021-02-02
页码8
关键词16S-trnI/trnA-23S regionantimicrobial peptidemicroparticle bombardmentNannochloropsis gaditanaplastid transformationribosome binding site
DOI10.1111/jpy.13099
通讯作者Cui, Yulin(yulincui@yic.ac.cn)
英文摘要The purpose of this study was to establish a plastid transformation system for expressing recombinant proteins in Nannochloropsis gaditana. On the basis of the sequenced plastid genome, the homologous flanking region, 16S-trnI/trnA-23S, and the endogenous regulatory fragments containing the psbA promoter, rbcL promoter, rbcL terminator, and psbA terminator were amplified from N. gaditana as elements of a plastid transformation vector. Then, the herbicide-resistant gene (bar) was used as a selectable marker, regulated by the psbA promoter and rbcL terminator. Finally, two codon-optimized antimicrobial peptide-coding genes linked by endogenous ribosome binding site (RBS) in a polycistron were inserted into the constructed vector under the regulation of the rbcL promoter and psbA terminator. After microparticle bombardment, the positive clones were detected using polymerase chain reaction (PCR), and Southern and Western blotting were used to assess the co-expression of the two antimicrobial peptides from the plastid. Nannochloropsis gaditana showed the potential to express recombinant proteins for biotechnological applications, for example, for the development of oral vaccines in aquaculture.
资助机构National Key Research and Development Program of China; National Natural Science Foundation of China; Natural Science Foundation of Shandong Province, China; Project of Innovation & Development of Marine Economy; Major Basic Research Program of Shandong Province Natural Science Foundation; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose)
收录类别SCI
语种英语
研究领域[WOS]Plant Sciences; Marine & Freshwater Biology
WOS记录号WOS:000613948500001
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27469
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Cui, Yulin作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China
3.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
4.Shanxi Agr Univ, Inst Mol Agr & Bioenergy, Taigu 030801, Peoples R China
推荐引用方式
GB/T 7714Cui, Yulin,Wang, Kang,Xu, Wenxin,et al. PLASTID ENGINEERING OF A MARINE ALGA, NANNOCHLOROPSIS GADITANA, FOR CO-EXPRESSION OF TWO RECOMBINANT PEPTIDES[J]. JOURNAL OF PHYCOLOGY,2021:8.
APACui, Yulin.,Wang, Kang.,Xu, Wenxin.,Wang, Yinchu.,Gao, Zhengquan.,...&Qin, Song.(2021).PLASTID ENGINEERING OF A MARINE ALGA, NANNOCHLOROPSIS GADITANA, FOR CO-EXPRESSION OF TWO RECOMBINANT PEPTIDES.JOURNAL OF PHYCOLOGY,8.
MLACui, Yulin,et al."PLASTID ENGINEERING OF A MARINE ALGA, NANNOCHLOROPSIS GADITANA, FOR CO-EXPRESSION OF TWO RECOMBINANT PEPTIDES".JOURNAL OF PHYCOLOGY (2021):8.
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