Liu, Wei1,2,3
; Bai, Xue1; Li, Yan1,2,3; Zhang, Haikun1,2,3; Hu, Xiaoke1,2,3发表期刊BMC MICROBIOLOGY
ISSN1471-2180
2021-03-09
卷号21期号:1页码:9
关键词Two-component response regulatorREC domainHTH_LuxR domainBacterial motilityExopolysaccharidesCompetitive nodulation
DOI10.1186/s12866-021-02138-w
通讯作者Hu, Xiaoke(xkhu@yic.ac.cn)
英文摘要BackgroundA wide variety of bacterial adaptative responses to environmental conditions are mediated by signal transduction pathways. Two-component signal transduction systems are one of the predominant means used by bacteria to sense the signals of the host plant and adjust their interaction behaviour. A total of seven open reading frames have been identified as putative two-component response regulators in the gram-negative nitrogen-fixing bacteria Azorhizobium caulinodans ORS571. However, the biological functions of these response regulators in the symbiotic interactions between A. caulinodans ORS571 and the host plant Sesbania rostrata have not been elucidated to date.ResultsIn this study, we identified and investigated a two-component response regulator, AcfR, with a phosphorylatable N-terminal REC (receiver) domain and a C-terminal HTH (helix-turn-helix) LuxR DNA-binding domain in A. caulinodans ORS571. Phylogenetic analysis showed that AcfR possessed close evolutionary relationships with NarL/FixJ family regulators. In addition, six histidine kinases containing HATPase_c and HisKA domains were predicted to interact with AcfR. Furthermore, the biological function of AcfR in free-living and symbiotic conditions was elucidated by comparing the wild-type strain and the Delta acfR mutant strain. In the free-living state, the cell motility behaviour and exopolysaccharide production of the Delta acfR mutant were significantly reduced compared to those of the wild-type strain. In the symbiotic state, the Delta acfR mutant showed a competitive nodule defect on the stems and roots of the host plant, suggesting that AcfR can provide A. caulinodans with an effective competitive ability for symbiotic nodulation.ConclusionsOur results showed that AcfR, as a response regulator, regulates numerous phenotypes of A. caulinodans under the free-living conditions and in symbiosis with the host plant. The results of this study help to elucidate the involvement of a REC+HTH_LuxR two-component response regulator in the Rhizobium-host plant interaction.
资助机构National Natural Science Foundation of China
收录类别SCI
语种英语
关键词[WOS]PROTEIN; SYSTEM
研究领域[WOS]Microbiology
WOS记录号WOS:000627445700002
引用统计被引频次：1[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27366
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Hu, Xiaoke作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Peoples R China
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Liu, Wei,Bai, Xue,Li, Yan,et al. FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant[J]. BMC MICROBIOLOGY,2021,21(1):9.
APALiu, Wei,Bai, Xue,Li, Yan,Zhang, Haikun,&Hu, Xiaoke.(2021).FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant.BMC MICROBIOLOGY,21(1),9.
MLALiu, Wei,et al."FixJ family regulator AcfR of Azorhizobium caulinodans is involved in symbiosis with the host plant".BMC MICROBIOLOGY 21.1(2021):9.
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