Wang, Xiaodong1; Miao, Xuexia2; Chen, Gao3; Cui, Yulin4
; Sun, Fengjie6; Fan, Jianhua5; Gao, Zhengquan1; Meng, Chunxiao1发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
2021-04-01
卷号54页码:10
关键词MicroRNAsAstaxanthinDeep sequencingHigh light stressSodium acetate stressTranscriptome
DOI10.1016/j.algal.2020.102179
通讯作者Gao, Zhengquan(gaozhengquan@sdut.edu.cn); Meng, Chunxiao(mengchunxiao@sdut.edu.cn)
英文摘要The growth and astaxanthin production of unicellular Haematococcus pluvialis is affected by both the high light and sodium acetate (NaAC) stresses. There are sparse on studies of the regulatory pathways of microRNAs (miRNAs) and their target genes in response to high light and NaAC stresses in H. pluvialis. In this study, transcriptomic analysis was used to identify the key target genes of miRNA regulation under both high light and NaAC stresses. A total of 434 (including 29 novel) miRNAs were identified, which were mainly enriched in metal ion binding and transport, and involved in basic metabolic pathways, such as pyruvate metabolism, ubiquitin mediated proteolysis, and oxidative phosphorylation. A total of 115 (out of 434) miRNAs were detected in response to high light and NaAC stresses. A total of 83 and 46 miRNAs significantly expressed under high light and NaAC stresses were considered as high light and NaAC stress responsive miRNAs, respectively. 14 miRNAs responded to both high light and NaAC stresses. Some miRNAs that regulate differentially expressed target genes had been identified. In the high light treatment group, 38 regulatory pairs were identified. In NaAc treatment group, 40 regulatory pairs were identified. These results shed light on the regulatory mechanisms of miRNAs responding to high light and NaAC stresses in H. pluvialis, further providing support for understanding the molecular mechanisms of miRNA regulation involved in astaxanthin synthesis in H. pluvialis.
资助机构National Natural Science Foundation of China; Natural Science Foundation of Shandong Province; Key Research and Development Program of Shandong Province (Food for Special Medical Purpose); Open Fund of Shandong Provincial Key Laboratory of Plant Stress
收录类别SCI
语种英语
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000632338300013
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/27324
专题海岸带生物学与生物资源利用重点实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
通讯作者Gao, Zhengquan; Meng, Chunxiao作者单位1.Shandong Univ Technol, Sch Life Sci, Zibo 255049, Peoples R China
2.Chinese Acad Sci, China Natl Ctr Bioinformat, Beijing Inst Genom, Key Lab Genom & Precis Med, Beijing 100101, Peoples R China
3.Shandong Acad Agr Sci, Biotechnol Res Ctr, Shandong Prov Key Lab Genet Improvement, Ecol & Physiol Crops, Jinan 250100, Peoples R China
4.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resource Utilizat, Yantai 264003, Peoples R China
5.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
6.Georgia Gwinnett Coll, Sch Sci & Technol, 1000 Univ Ctr Lane, Lawrenceville, GA 30043 USA
推荐引用方式
GB/T 7714Wang, Xiaodong,Miao, Xuexia,Chen, Gao,et al. Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2021,54:10.
APAWang, Xiaodong.,Miao, Xuexia.,Chen, Gao.,Cui, Yulin.,Sun, Fengjie.,...&Meng, Chunxiao.(2021).Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,54,10.
MLAWang, Xiaodong,et al."Identification of microRNAs involved in astaxanthin accumulation responding to high light and high sodium acetate (NaAC) stresses in Haematococcus pluvialis".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 54(2021):10.
PDF全文下载地址:
点我下载PDF
