Liu, Ping; Li, Bowei
; Fu, Longwen; Huang, Yan; Man, Mingsan; Qi, Ji; Sun, Xiyan; Kang, Qi; Shen, Dazhong; Chen, Lingxin发表期刊ACS SENSORS
ISSN2379-3694
2020-02
卷号5期号:2页码:464-473
关键词INFRARED FLUORESCENT-PROBEHYDROGEN-SULFIDENITRIC-OXIDESULFANE SULFURCANCER-CELLSCULTUREGRADIENTSSCAFFOLDSCHIPHNO
研究领域Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology
DOI10.1021/acssensors.9b02205
产权排序[Liu, Ping; Li, Bowei; Fu, Longwen; Huang, Yan; Man, Mingsan; Qi, Ji; Sun, Xiyan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Liu, Ping; Li, Bowei; Fu, Longwen; Huang, Yan; Man, Mingsan; Qi, Ji; Sun, Xiyan; Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Megasci, Yantai 264003, Peoples R China; [Liu, Ping; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Key Lab Mol & Nano Probes, Minist Educ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China; [Huang, Yan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
通讯作者Li, Bowei(bwli@yic.ac.cn); Shen, Dazhong(dzshen@sdnu.edu.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要In this paper, we first proposed a novel hybrid three-dimensional (3D) printed and paper-based microfluidic platform and applied it for investigating the cell's apoptosis and intracellular cross-talk. The fabrication of a 3D-printed microfluidic chip is much easier than polydimethylsiloxane (PDMS) chip and can be applied in many common labs without soft lithogrophy fabrication equipment. Moreover, 3D printing can be perfectly combined with paper-based chips that can provide 3D scaffold for cell culture and analysis. In addition, these paper chips are disposable after use, greatly reducing the experimental cost. We integrated "Christmas Tree" structure with the top layer of the 3D-printed microfluidic chip to generate a continuous concentration gradient, and the bottom layer contained paper-based chips as cell culture area. The two-layer structure allows the concentration gradient forming layer to be separated from the cell culture layer, which can simplify the planting of cells in the microfluidic chip and make sure the cells stay in the culture chambers and don't clog the microfluidic channels. Applying this hybrid platform, we examined the effect of H2S on cancer cells. Continuous exposure to a low concentration of H2S inhibited cancer cell SMMC-7721 proliferation by inducing cell apoptosis. We also found that two gaseous molecules H2S and NO have cross-talk in cancer cells; they formed bioactive intermediate polysulfides in cancer cells. It is expected that this novel hybrid 3D-printed and paper-based microfluidic platform will have widespread application prospects in cell investigation.
文章类型Article
资助机构National Key Research and Development Program of China [2016YFC1400702]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 21976209, 21874083, 81873697]; Research Initiation Fund of Binzhou Medical University [BY2019KYQD39]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars; Taishan Scholar Project Special Funding [ts20190962]
收录类别SCI
语种英语
关键词[WOS]INFRARED FLUORESCENT-PROBE; HYDROGEN-SULFIDE; NITRIC-OXIDE; SULFANE SULFUR; CANCER-CELLS; CULTURE; GRADIENTS; SCAFFOLDS; CHIP; HNO
研究领域[WOS]Chemistry; Science & Technology - Other Topics
WOS记录号WOS:000517856700023
引用统计被引频次：16[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/25091
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Ctr Ocean Megasci, Yantai 264003, Peoples R China;
3.Shandong Normal Univ, Key Lab Mol & Nano Probes, Minist Educ, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Peoples R China;
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Liu, Ping,Li, Bowei,Fu, Longwen,et al. Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk[J]. ACS SENSORS,2020,5(2):464-473.
APALiu, Ping.,Li, Bowei.,Fu, Longwen.,Huang, Yan.,Man, Mingsan.,...&Chen, Lingxin.(2020).Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk.ACS SENSORS,5(2),464-473.
MLALiu, Ping,et al."Hybrid Three Dimensionally Printed Paper-Based Microfluidic Platform for Investigating a Cell's Apoptosis and Intracellular Cross-Talk".ACS SENSORS 5.2(2020):464-473.
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