Wen, Jiahui; Liu, Yongming; Li, Jingwen; Lin, Hao; Zheng, Yiran; Chen, Yan; Fu, Xiuli; Chen, Lingxin
发表期刊ANALYST
ISSN0003-2654
2020-04-07
卷号145期号:7页码:2774-2778
关键词SINGLE-STRANDED-DNATURN-ON DETECTIONCOPPER NANOPARTICLESENZYMATIC CLEAVAGEOXIDATIVE DAMAGEASSAYFLUORESCENCEINHIBITIONGOLDUNIVERSAL
研究领域Chemistry, Analytical
DOI10.1039/d0an00060d
产权排序[Wen, Jiahui; Liu, Yongming; Li, Jingwen; Lin, Hao; Zheng, Yiran; Fu, Xiuli] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; [Chen, Yan] Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
通讯作者Fu, Xiuli(xlfu@yic.ac.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要A label-free, sensitive, simple and general colorimetric method was reported to monitor S1 nuclease activity based on protamine-assisted aggregation of gold nanoparticles (AuNPs). Here, protamine, a linear polycation, was used as a medium for causing the aggregation of negatively charged AuNPs by electrostatic interactions, resulting in changes in the surface plasmon resonance (SPR) absorption bands as well as the color of AuNPs. Here, the AuNPs were employed as an indicator to detect the level of S1 nuclease in the solution. Substrate DNA could be cleaved into small fragments by the specific S1 nuclease, which effectively prevents the electrostatic interaction between DNA and protamine and thus facilitates the interaction between protamine and AuNPs. The quantitative analysis of S1 nuclease activity can be performed via directly measuring the changes in the absorption spectra of the AuNPs. Using S1 nuclease as a model analyte, the limit of detection was estimated to be 1.0 x 10(-4) U mL(-1). Furthermore, the proposed concept has been successfully applied in S1 nuclease analysis of serum samples, offering an ultrasensitive strategy for the speedy detection of the nuclease activity and providing a new avenue for high-throughput screening of nucleases and drugs with potential inhibition properties.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 61801274, 21976209]; Natural Science Foundation of Shandong Province in ChinaNatural Science Foundation of Shandong Province [ZR2017BB026]; Taishan Scholar Project Special Funding [ts20190962]
收录类别SCI
语种英语
关键词[WOS]SINGLE-STRANDED-DNA; TURN-ON DETECTION; COPPER NANOPARTICLES; ENZYMATIC CLEAVAGE; OXIDATIVE DAMAGE; ASSAY; FLUORESCENCE; INHIBITION; GOLD; UNIVERSAL
研究领域[WOS]Chemistry
WOS记录号WOS:000526825700033
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/25053
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China;
2.Shandong Agr & Engn Univ, Sch Resources & Environm Engn, Jinan 250100, Peoples R China;
3.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
4.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Wen, Jiahui,Liu, Yongming,Li, Jingwen,et al. A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity[J]. ANALYST,2020,145(7):2774-2778.
APAWen, Jiahui.,Liu, Yongming.,Li, Jingwen.,Lin, Hao.,Zheng, Yiran.,...&Chen, Lingxin.(2020).A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity.ANALYST,145(7),2774-2778.
MLAWen, Jiahui,et al."A label-free protamine-assisted colorimetric sensor for highly sensitive detection of S1 nuclease activity".ANALYST 145.7(2020):2774-2778.
PDF全文下载地址:
点我下载PDF
