Wang, Xiaoyan; Yu, Shuangmei; Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen
; Li, Bowei; Li, Jinhua; Chen, Lingxin发表期刊TALANTA
ISSN0039-9140
2020-05-01
卷号211页码:120727
关键词RATIOMETRIC FLUORESCENCEOPTICAL-DETECTIONGLYCOPROTEINAFFINITYRECOGNITIONSEPARATIONSTRATEGYSENSORPH
研究领域Chemistry, Analytical
DOI10.1016/j.talanta.2020.120727
产权排序[Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Wang, Jiangru; Yu, Jialuo; Arabi, Maryam; Fu, Longwen; Li, Bowei; Li, Jinhua; Chen, Lingxin] Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Yu, Shuangmei] Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
通讯作者Wang, Xiaoyan(wangxy@yic.ac.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要We reported a facile strategy to assemble a ratiometric nanosensor for the ovalbumin (OVA) fluorescence determination and meanwhile it can be utilized for selective visual identification by naked eyes with fluorescent test papers under 365 nm UV lamp. The nanosensor was prepared through simply mixing blue color carbon dots (CDs) and green color core-shell imprinted polymers. Blue CDs were used directly as the internal reference without participating in the imprinting process and modified molecularly imprinted polymers (MIPs) were synthesized by post-imprinting, using fluorescein isothiocyanate (FITC) as fluorescence enhanced signal. Upon the addition of different concentrations of OVA, the fluorescence intensity of FITC was enhanced, while the fluorescence intensity of CDs was almost unchanged, leading to a detection limit as low as 15.4 nM. Accordingly, the fluorescence color was gradually changed from blue to dark olive green to green with naked eyes observation. Moreover, the ratiometric nanosensor was successfully applied to detect OVA in the human urine samples with satisfactory recoveries attaining of 92.0-104.0% with relative standard deviation (RSD) of 3.3-3.9% and 93.3-101.0% with RSDs of 2.7-3.8% for the spiked chicken egg white samples. This strategy reported here opens a novel pathway for biomacromolecule detection in real applications and can realize the visual observation on fluorescent test papers.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21804010, 21575159, 41776110, 21876199, 21976209]; Science and Technology Development Plan of Shandong Province of China [2014GGF01074]; Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050]; Research Initiation Fund of Binzhou Medical University [BY2019KYQD39]; Taishan Scholar Project Special Funding [ts20190962]
收录类别SCI
语种英语
关键词[WOS]RATIOMETRIC FLUORESCENCE; OPTICAL-DETECTION; GLYCOPROTEIN; AFFINITY; RECOGNITION; SEPARATION; STRATEGY; SENSOR; PH
研究领域[WOS]Chemistry
WOS记录号WOS:000518708300036
引用统计被引频次：19[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/25039
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
2.Chinese Cademy Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Yantai Yuhuangding Hosp, Radiotherapy Ward, Yantai 264000, Peoples R China
推荐引用方式
GB/T 7714Wang, Xiaoyan,Yu, Shuangmei,Wang, Jiangru,et al. Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin[J]. TALANTA,2020,211:120727.
APAWang, Xiaoyan.,Yu, Shuangmei.,Wang, Jiangru.,Yu, Jialuo.,Arabi, Maryam.,...&Chen, Lingxin.(2020).Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin.TALANTA,211,120727.
MLAWang, Xiaoyan,et al."Fluorescent nanosensor designing via hybrid of carbon dots and post- imprinted polymers for the detection of ovalbumin".TALANTA 211(2020):120727.
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