Jeon, J; Choi, N; Chen, H; Moon, JI; Chen, LX
; Choo, J发表期刊LAB ON A CHIP
ISSN1473-0197
2019-02-21
卷号19期号:4页码:674-681
关键词ENHANCED RAMAN-SCATTERINGGOLD NANOFLOWERSIMMUNOASSAYPLATFORMS
研究领域Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology; Instruments & Instrumentation
DOI10.1039/c8lc01180j
产权排序[Jeon, Jinhyeok; Choi, Namhyun; Chen, Hao; Moon, Joung-Il] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea; [Chen, Hao; Moon, Joung-Il; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要In the last two decades, microfluidic technology has emerged as a highly efficient tool for the study of various chemical and biological reactions. Recently, we reported that high-throughput detection of various concentrations of a reagent is possible using a continuous gradient microfluidic channel combined with a surface-enhanced Raman scattering (SERS) detection platform. In this continuous flow regime, however, the deposition of nanoparticle aggregates on channel surfaces induces the memory effect, affecting both sensitivity and reproducibility. To resolve this problem, a SERS-based gradient droplet system was developed. Herein, the serial dilution of a reagent was achieved in a stepwise manner using microfluidic concentration gradient generators. Then various concentrations of a reagent generated in different channels were simultaneously trapped into the tiny volume of droplets by injecting an oil mixture into the channel. Compared to the single-phase regime, this two-phase liquid/liquid segmented flow regime allows minimization of resident time distributions of reagents through localization of reagents in encapsulated droplets. Consequently, the sample stacking problem could be solved using this system because it greatly reduces the memory effect. We believe that this SERS-based gradient droplet system will be of significant utility in simultaneously monitoring chemical and biological reactions for various concentrations of a reagent.
文章类型Article
资助机构National Research Foundation of KoreaNational Research Foundation of Korea [2017M3D1A1039287, 2018M3A7B4071203]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 21575159]
收录类别SCI
语种英语
关键词[WOS]ENHANCED RAMAN-SCATTERING; GOLD NANOFLOWERS; IMMUNOASSAY; PLATFORMS
研究领域[WOS]Biochemical Research Methods; Chemistry, Multidisciplinary; Chemistry, Analytical; Nanoscience & Nanotechnology; Instruments & Instrumentation
WOS记录号WOS:000459726400011
引用统计被引频次：39[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24994
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea;
2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Jeon, J,Choi, N,Chen, H,et al. SERS-based droplet microfluidics for high-throughput gradient analysis[J]. LAB ON A CHIP,2019,19(4):674-681.
APAJeon, J,Choi, N,Chen, H,Moon, JI,Chen, LX,&Choo, J.(2019).SERS-based droplet microfluidics for high-throughput gradient analysis.LAB ON A CHIP,19(4),674-681.
MLAJeon, J,et al."SERS-based droplet microfluidics for high-throughput gradient analysis".LAB ON A CHIP 19.4(2019):674-681.
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