Li, WJ; Jiang, TT; Pu, Y; Jiao, XD
; Tan, WQ; Qin, S发表期刊MICRO & NANO LETTERS
ISSN1750-0443
2019-03-06
卷号14期号:3页码:344-348
关键词biochemistrybiosensorsnanosensorsnanofabricationfluorescencemolecular biophysicsradiation quenchingchemical sensorssugarproteinsbiomedical optical imaginggraphene compoundsGO-CSlow concentrationscompetitive bindingmaltose-binding proteinrecombinant phycocyaninfar-red fluorescenceMBP-rPCrPC emissionrPC fluorescencelinear detection rangeglucose biosensorfluorescence quenchingchitosan-modified graphene oxidelow-molecular-weight chitosanglucose detectionfluorescence resonance energy transfergraphene oxide nanomaterialmass 01 mg to 10 mgCO
研究领域Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
DOI10.1049/mnl.2018.5269
产权排序[Li, Wenjun; Jiao, Xudong; Tan, Wenqiang; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [Jiang, Tingting] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Pu, Yang] Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要A novel graphene oxide (GO) nanomaterial, which was rapidly and simply synthesised and conjugated with low-molecular-weight chitosan (CS), was used to develop a simple, sensitive GO biosensor system for glucose detection by fluorescence resonance energy transfer. The GO-CS was used to detect low concentrations of glucose based on competitive binding with maltose-binding protein (MBP). The -subunit of recombinant phycocyanin (rPC), which emits far-red fluorescence, was used to label MBP (MBP-rPC). The rPC emission was quenched by binding between GO-CS and MBP. However, in the presence of glucose, GO-CS was out-competed for binding to MBP, leading to rPC fluorescence. Glucose was sensitively and selectively detected with this biosensor, with a linear detection range of 0.1-1 mg glucose/ml. The limit of detection for glucose was approximate to 0.05 mg/ml.
文章类型Article
资助机构Marine Economy Innovation Development Demonstration Project State Oceanic Administration of China [YHCX-SW-P-201701, YHCX-SW-Y-201701]; Scientific Research Foundation of Ludong University [ly2014041]
收录类别SCI
语种英语
关键词[WOS]FRET BIOSENSOR; PURIFICATION; MANAGEMENT; PROTEIN; SENSOR; DNA
研究领域[WOS]Nanoscience & Nanotechnology; Materials Science, Multidisciplinary
WOS记录号WOS:000461572800025
引用统计被引频次：5[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24977
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
2.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China;
3.Ludong Univ, Sch Agr, Yantai 264025, Peoples R China
推荐引用方式
GB/T 7714Li, WJ,Jiang, TT,Pu, Y,et al. Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide[J]. MICRO & NANO LETTERS,2019,14(3):344-348.
APALi, WJ,Jiang, TT,Pu, Y,Jiao, XD,Tan, WQ,&Qin, S.(2019).Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide.MICRO & NANO LETTERS,14(3),344-348.
MLALi, WJ,et al."Glucose biosensor using fluorescence quenching with chitosan-modified graphene oxide".MICRO & NANO LETTERS 14.3(2019):344-348.
PDF全文下载地址:
点我下载PDF
