Fu, XL; Wang, YQ
; Liu, YM; Liu, HT; Fu, LW; Wen, JH; Li, JW; Wei, PH; Chen, LX发表期刊ANALYST
ISSN0003-2654
2019-03-07
卷号144期号:5页码:1582-1589
关键词ACUTE MYOCARDIAL-INFARCTIONDISPERSED CARBON NANOTUBESPEROXIDASE-LIKE CATALYSISSURFACE-ENHANCED RAMANULTRASENSITIVE DETECTIONCOLORIMETRIC SENSOROPTICAL NANOPROBESSILVER DEPOSITIONASSAYBIOSENSORS
研究领域Chemistry, Analytical
DOI10.1039/c8an02022a
产权排序[Fu, Xiuli; Liu, Yongming; Liu, Huitao; Wen, Jiahui; Li, Jingwen; Chen, Lingxin] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; [Wang, Yunqing; Fu, Longwen; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wei, Peihai] Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
作者部门海岸带环境工程技术研究与发展中心
英文摘要Cardiac troponin I (cTnI) was considered as the gold standard for acute myocardial infarction (AMI) diagnosis owing to its superior cardiac specificity for cardiac damage and showing little or no changes in patients with a skeletal muscle disease or trauma. Herein, a new signal amplification surface-enhanced Raman scattering (SERS) platform was developed for recognition and detection of cTnI by using gold nanoparticles (AuNPs), graphene oxide (GO) and magnetic beads (MB). Here, antibody/Raman reporter labeled AuNP-functionalized GO were employed as both SERS nanotags and signal amplification carriers. Monoclonal antibody modified MB were applied as the capture probe and separation agents. In the presence of cTnI, sandwich type immunocomplexes, capture probe/target/SERS nanotags, were formed through antibody-antigen-antibody interactions. Due to the strong SERS enhancement ability of the designed GO/AuNP complexes and a high binding chance between cTnI and the GO/AuNP complexes, the proposed SERS-based immunoassay could selectively detect cTnI with a high sensitivity (detection limit of 5 pg mL(-1)) and a good linearity was obtained in a range of 0.01-1000 ng mL(-1). In addition, this method was also successfully applied for detecting cTnI in serum substitute media with a similar linear range. Furthermore, this strategy can be constructed with different kinds of antibodies and Raman reporters, and thus can be easily used for simultaneous detection of multiple biomarkers. Therefore, this proposed SERS-based signal amplification technique shows strong potential for the clinical diagnosis of AMI disease.
文章类型Article
资助机构National Natural Science Foundation of Shandong Province in China [ZR2017BB026]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21275158, 21575159]; Shandong Provincial Natural Science FoundationNatural Science Foundation of Shandong Province [ZR2014BL031]
收录类别SCI
语种英语
关键词[WOS]ACUTE MYOCARDIAL-INFARCTION; DISPERSED CARBON NANOTUBES; PEROXIDASE-LIKE CATALYSIS; SURFACE-ENHANCED RAMAN; ULTRASENSITIVE DETECTION; COLORIMETRIC SENSOR; OPTICAL NANOPROBES; SILVER DEPOSITION; ASSAY; BIOSENSORS
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000461614000008
引用统计被引频次：52[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24976
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Qilu Normal Univ, Sch Chem & Chem Engn, 36 Lishan Rd, Jinan 250013, Shandong, Peoples R China
推荐引用方式
GB/T 7714Fu, XL,Wang, YQ,Liu, YM,et al. A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I[J]. ANALYST,2019,144(5):1582-1589.
APAFu, XL.,Wang, YQ.,Liu, YM.,Liu, HT.,Fu, LW.,...&Chen, LX.(2019).A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I.ANALYST,144(5),1582-1589.
MLAFu, XL,et al."A graphene oxide/gold nanoparticle-based amplification method for SERS immunoassay of cardiac troponin I".ANALYST 144.5(2019):1582-1589.
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