He, N; Bai, S; Huang, Y; Xing, YL; Chen, LX
; Yu, FB; Lv, CJ发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2019-04-16
卷号91期号:8页码:5424-5432
关键词DESIGN STRATEGIESOXIDATIVE STRESSMOLECULAR DESIGNSUBSTRATEAGGREGATIONINJURYSITESA1-1
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.9b00713
产权排序[He, Na; Bai, Song; Lv, Changjun] Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China; [Huang, Yan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Xing, Yanlong; Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Key Lab Hainan Trauma & Disaster Rescue, Inst Funct Mat & Mol Imaging,Coll Clin med, Haikou 571199, Hainan, Peoples R China; [He, Na; Bai, Song; Lv, Changjun] Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China
通讯作者Chen, Lingxin(lxchen@yic.ac.cn); Yu, Fabiao(fbyu@yic.ac.cn); Lv, Changjun(lucky_lcj@sina.com)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Idiopathic pulmonary fibrosis (IPF) is a lung-limited and progressive fibrotic disease. The early diagnosis and therapies of IPF are still full of clinical challenges. Glutathione S-transferase (GSTs) plays significant roles in promoting the formation of pulmonary fibrosis. Herein, we report a fluorescent probe (Cy-GST) for the detection of GSTs concentration fluctuations in cells and in mice models. The probe can selectively and sensitively respond to GSTs with an "off-on" type fluorescence switch. Our results demonstrated that the level of intracellular GSTs increase in the pulmonary fibrosis cells and mice models. And the IPF patients hold high levels of GSTs concentrations. Thus, GSTs are likely to play important roles in pulmonary fibrosis. The inhibitor of GSTs TLK117 can reduce the severity of pulmonary fibrosis. The synergistic treatment of TLK117 and pirfenidone have better therapeutic effects than only using pirfenidone in pulmonary fibrosis mice models. The level of GSTs in IPF may be a new potential marker for IPF diagnosis. And the inhibition of GSTs may be a new therapeutic strategy for IPF treatment.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21775162, 21864011, 81670064, 81870001]; Talent Program of Hainan Medical University [XRC180006, XRC180007]; Medical Science and Technology Development Project of Shandong Province [2017WS800]
收录类别SCI
语种英语
关键词[WOS]DESIGN STRATEGIES; OXIDATIVE STRESS; MOLECULAR DESIGN; SUBSTRATE; AGGREGATION; INJURY; SITES; A1-1
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000465189600066
引用统计被引频次：20[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24957
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Binzhou Med Univ Hosp, Dept Resp Med, Binzhou 256603, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Hainan Med Univ, Coll Emergency & Trauma, Key Lab Hainan Trauma & Disaster Rescue, Inst Funct Mat & Mol Imaging,Coll Clin med, Haikou 571199, Hainan, Peoples R China;
4.Binzhou Med Univ, Inst Mol Med, Med Res Ctr, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714He, N,Bai, S,Huang, Y,et al. Evaluation of Glutathione S-Transferase Inhibition Effects on Idiopathic Pulmonary Fibrosis Therapy with a Near-Infrared Fluorescent Probe in Cell and Mice Models[J]. ANALYTICAL CHEMISTRY,2019,91(8):5424-5432.
APAHe, N.,Bai, S.,Huang, Y.,Xing, YL.,Chen, LX.,...&Lv, CJ.(2019).Evaluation of Glutathione S-Transferase Inhibition Effects on Idiopathic Pulmonary Fibrosis Therapy with a Near-Infrared Fluorescent Probe in Cell and Mice Models.ANALYTICAL CHEMISTRY,91(8),5424-5432.
MLAHe, N,et al."Evaluation of Glutathione S-Transferase Inhibition Effects on Idiopathic Pulmonary Fibrosis Therapy with a Near-Infrared Fluorescent Probe in Cell and Mice Models".ANALYTICAL CHEMISTRY 91.8(2019):5424-5432.
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