Huang, Y; He, N; Wang, YD; Zhang, LW
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发表期刊JOURNAL OF MATERIALS CHEMISTRY B
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ISSN2050-750X
2019-04-21
卷号7期号:15页码:2557-2564
关键词OXIDATIVE STRESSIMAGING BASALLIVE-CELLTURN-ONMITOCHONDRIAHCLOANTIOXIDANTSZEBRAFISHLYSOSOME
研究领域Materials Science, Biomaterials
DOI10.1039/c9tb00079h
产权排序[Huang, Yan; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Shandong, Peoples R China; [Huang, Yan; He, Na; Wang, Yude; Zhang, Liangwei; Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China; [He, Na; Wang, Yude] Binzhou Med Univ, Med Res Ctr, Inst Mol Med, Yantai 264003, Peoples R China; [Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
通讯作者Kang, Qi(kangqi@sdnu.edu.cn); Choo, Jaebum(jbchoo@cau.ac.kr); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Hypoxic stress is a common concern in medicine and biology, which can induce the cellular injury and death by excess production of reactive oxygen species (ROS). Hypochlorous acid (HOCl), one of the ROS, plays a crucial role in the oxidative damage to tissue proteins in the pathogenesis of various diseases. Therefore, excess production of HOCl might be an important factor for the damage caused by hypoxic stress. However, most of the present methods cannot meet the demand of real-time detection on account of the labile and reactive chemical properties of HOCl. Herein, we designed a near-infrared fluorescent probe, Cy-HOCl, for the selective imaging of HOCl in cells and in vivo. Cy-HOCl includes two moieties: a 4-amino-3-nitrophenol group as the response unit and a near-infrared heptamethine cyanine fluorophore as the fluorescent modulator. Cy-HOCl exhibits excellent selectivity and sensitivity towards the detection of HOCl. The hypoxic response behavior of Cy-HOCl is evaluated in cells to clarify the relationship between HOCl and hypoxia. The probe is also applied to measure HOCl in ex vivo-dissected organs of an acute ischemia mouse model as well as for real-time monitoring the changes of HOCl in the hypoxic zebrafish model.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 81573393, 21575080]; program of Youth Innovation Promotion Association, CAS [2017256, 2019217]; National Research Foundation of KoreaNational Research Foundation of Korea [2017M3D1A1039287, 2018M3A7B4071203]; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
收录类别SCI
语种英语
关键词[WOS]OXIDATIVE STRESS; IMAGING BASAL; LIVE-CELL; TURN-ON; MITOCHONDRIA; HCLO; ANTIOXIDANTS; ZEBRAFISH; LYSOSOME
研究领域[WOS]Materials Science, Biomaterials
WOS记录号WOS:000465281100014
引用统计被引频次:14[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24955
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Shandong Normal Univ, Coll Chem Chem Engn & Mat Sci, Key Lab Mol & Nano Probes, Minist Educ, Jinan 250014, Shandong, Peoples R China;
2.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China;
3.Binzhou Med Univ, Med Res Ctr, Inst Mol Med, Yantai 264003, Peoples R China;
4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea;
5.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Peoples R China
推荐引用方式
GB/T 7714Huang, Y,He, N,Wang, YD,et al. Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress[J]. JOURNAL OF MATERIALS CHEMISTRY B,2019,7(15):2557-2564.
APAHuang, Y.,He, N.,Wang, YD.,Zhang, LW.,Kang, Q.,...&Chen, LX.(2019).Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress.JOURNAL OF MATERIALS CHEMISTRY B,7(15),2557-2564.
MLAHuang, Y,et al."Detection of hypochlorous acid fluctuation via a selective near-infrared fluorescent probe in living cells and in vivo under hypoxic stress".JOURNAL OF MATERIALS CHEMISTRY B 7.15(2019):2557-2564.
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