Wang, WJ; Sheng, YQ
发表期刊ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS
ISSN2211-9264
2019-06
卷号40页码:101490
关键词BacteriaHigh-throughput SequencingMetabolomicsPhotomicrobial Fuel CellsSynechocystis
研究领域Biotechnology & Applied Microbiology
DOI10.1016/j.algal.2019.101490
产权排序[Sheng, Yanqing] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China
; Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
通讯作者Sheng, Yanqing(yqsheng@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Photomicrobial fuel cells (pMFCs) are a new technology for transforming the light energy or bioenergy of algae into bioelectricity. However, the current methods of generating algal pMFCs have poor efficiency due to the complex electron transfer network in algal cells. In this study, pMFCs containing the model electricity-producing cyanobacterium Synechocystis sp. PCC6803 contaminated by additional bacteria exhibited a 12.6-fold greater voltage output compared to the control pMFCs with axenic Synechocystis sp. PCC6803. To explain this phenomenon, the compositions of symbiotic bacterial communities in bacterial-contaminated pMFCs were analyzed, and a pure strain WJ04 belonging to the genus Pseudomonas, which was the second most abundant (30.2%) among the total symbiotic bacteria, was obtained. The strain WJ04 was able to raise the voltage output by 8.3 fold compared with the control treatments, meaning that strain WJ04 contributed to the increased voltage output of pMFCs. Through the coculture experiment, strain WJ04 was found to influence the growth of Synechocystis, improve photosynthesis rates by regulating algal genes and further affect algal metabolite production compared with control treatments. In addition, nicotinamide, c-homocysteic acid and 4-aminobutyric acid (fold change analysis, F.C. >= 10) could regulate algal gene expression and enhance the coculture current density by 6.1-12.3 fold compared with the control treatments. Thus, these results provide a clear explanation for the high current production in bacterial-contaminated pMFCs, clarify the interaction mechanisms between algae and bacteria in the anode and provide accurate evidence for the role of bacteria in improving algal bioelectricity utilization.
文章类型Article
资助机构National Natural Science Foundation of China; Science and Technology Plan of Yantai City; Strategic Priority Research Program of the Chinese Academy of Sciences
收录类别SCI
语种英语
关键词[WOS]Electron-transfer; Bacteria; Aeruginosa; Oxidases; Algae; Acid
研究领域[WOS]Biotechnology & Applied Microbiology
WOS记录号WOS:000468144100014
引用统计被引频次：3[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24933
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc, Yantai 264003, Peoples R China;
2.Chinese Acad Sci, Ctr Ocean Megasci, 7 Nanhai Rd, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Wang, WJ,Sheng, YQ. Pseudomonas sp. strain WJ04 enhances current generation of Synechocystis sp. PCC6803 in photomicrobial fuel cells[J]. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,2019,40:101490.
APAWang, WJ,&Sheng, YQ.(2019).Pseudomonas sp. strain WJ04 enhances current generation of Synechocystis sp. PCC6803 in photomicrobial fuel cells.ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS,40,101490.
MLAWang, WJ,et al."Pseudomonas sp. strain WJ04 enhances current generation of Synechocystis sp. PCC6803 in photomicrobial fuel cells".ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS 40(2019):101490.
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