Gao, M
; Zhang, X; Wang, Y; Liu, QL; Yu, FB; Huang, Y; Ding, CF; Chen, LX发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2019-06-18
卷号91期号:12页码:7774-7781
关键词REACTIVE OXYGENSULFANE SULFURLIVING CELLSOXIDATIVE STRESSCHEMICAL PROBESON PROBESULFIDENITROREDUCTASELUMINESCENTPEROXYNITRITE
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.9b01189
产权排序[Gao, Min; Zhang, Xia; Wang, Yue; Huang, Yan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Yu, Fabiao] Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China; [Liu, Qingluan] China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China; [Ding, Caifeng] Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China; [Ding, Caifeng] Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China; [Gao, Min; Zhang, Xia; Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
通讯作者Yu, Fabiao(fbyu@yic.ac.cn); Ding, Caifeng(dingcaifeng@qust.edu.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Chronic hypoxic stress disrupts the intracellular redox homeostasis, leads to a series of physiological dysfunction, and finally results in many diseases including cancer and inflammatory and cardiovascular diseases. The intracellular redox status is related to the homeostasis between reactive oxygen species (ROS) and cellular antioxidant species. Superoxide anion (O-2(center dot-) is considered to be a precursor of ROS. As a member of reactive sulfur species, hydrogen polysulfides (H2Sn) are a class of antioxidants in cells, which act as an important regulator for the intracellular redox state. Therefore, trapping the cross-talk of O-2(center dot-) and H2Sn is a benefit for further understanding the physiological and pathological effects. Herein, we conceive a fluorescent probe HCy-ONO for sequential detection of O-2(center dot-) and H2Sn in cells and in mouse models. Based on a tandem reaction, the probe HCy-ONO can be used to detect O-2(center dot-) and H2Sn in different fluorescence collection windows without spectral overlap interference with limits of detection 90 and 100 nM, respectively. The strategy affords high sensitivity and selectivity for our detection in living cell models under continuous hypoxic and intermittent hypoxic conditions, revealing the reason for ischemia-reperfusion injury. Moreover, the probe can distinguish the inflamed tissue from normal tissue in acute peritonitis mouse model. Finally, our probe is successfully applied for imaging of O-2(center dot-) and H2Sn in the SH-SYSY tumor-bearing mouse model, which is helpful to elucidate the physiological and pathological processes. These data demonstrated that different hypoxic status lead to different concentrations between H2Sn and O-2(center dot-).
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 21775162, 41776110]; State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, CASChinese Academy of Sciences [KF2016-22]; Key Laboratory of Sensor Analysis of Tumor Marker Ministry of Education, Qingdao University of Science and Technology [SATM201705]
收录类别SCI
语种英语
关键词[WOS]REACTIVE OXYGEN; SULFANE SULFUR; LIVING CELLS; OXIDATIVE STRESS; CHEMICAL PROBES; ON PROBE; SULFIDE; NITROREDUCTASE; LUMINESCENT; PEROXYNITRITE
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000472682000037
引用统计被引频次：20[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24918
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
中科院海岸带环境过程与生态修复重点实验室_污染过程与控制实验室
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Hainan Med Univ, Coll Emergency & Trauma, Inst Funct Mat & Mol Imaging, Haikou 571199, Hainan, Peoples R China;
3.China Med Univ, Div Clin Med 3, Shenyang 110122, Liaoning, Peoples R China;
4.Qingdao Univ Sci & Technol, MOE, Key Lab Opt Electr Sensing & Analyt Chem Life Sci, Qingdao 266042, Shandong, Peoples R China;
5.Qingdao Univ Sci & Technol, Coll Chem & Mol Engn, Qingdao 266042, Shandong, Peoples R China;
6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
7.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Gao, M,Zhang, X,Wang, Y,et al. Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative[J]. ANALYTICAL CHEMISTRY,2019,91(12):7774-7781.
APAGao, M.,Zhang, X.,Wang, Y.,Liu, QL.,Yu, FB.,...&Chen, LX.(2019).Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative.ANALYTICAL CHEMISTRY,91(12),7774-7781.
MLAGao, M,et al."Sequential Detection of Superoxide Anion and Hydrogen Polysulfides under Hypoxic Stress via a Spectral-Response-Separated Fluorescent Probe Functioned with a Nitrobenzene Derivative".ANALYTICAL CHEMISTRY 91.12(2019):7774-7781.
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