Ji, CL
; Lu, Z; Xu, LL; Li, F; Cong, M; Shan, XJ; Wu, HF发表期刊ENVIRONMENTAL POLLUTION
ISSN0269-7491
2019-08
卷号251页码:802-810
关键词CadmiumiTRAQProteomicsMitochondrial toxicityRuditapes philippinarum
研究领域Environmental Sciences
DOI10.1016/j.envpol.2019.05.046
产权排序[Ji, Chenglong; Lu, Zhen; Xu, Lanlan; Li, Fei; Cong, Ming; Wu, Huifeng] Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Shan, Xiujuan; Wu, Huifeng] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China; [Lu, Zhen; Xu, Lanlan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Wu, Huifeng(hfwu@yic.ac.cn)
作者部门海岸带环境过程实验室
英文摘要Cadmium is one of the most serious metal pollutants in the Bohai Sea. Previous studies revealed that mitochondrion might be the target organelle of Cd toxicity. However, there is a lack of a global view on the mitochondrial responses in marine animals to Cd. In this work, the mitochondrial responses were characterized in clams Ruditapes philippinarum treated with two concentrations (5 and 50 mu g/L) of Cd for 5 weeks using tetraethylbenzimidazolylcarbocyanine iodide (JC-1) staining, ultrastructural observation and quantitative proteomic analysis. Basically, a significant decrease of mitochondrial membrane potential (Delta Psi m) was observed in clams treated with the high concentration (50 mu g/L) of Cd. Cd treatments also induced specific morphological changes indicated by elongated mitochondria. Furthermore, iTRAQ-based mitochondrial proteomics showed that a total of 97 proteins were significantly altered in response to Cd treatment. These proteins were closely associated with multiple biological processes in mitochondria, including tricarboxylic acid (TCA) cycle, oxidative phosphorylation, fatty acid beta-oxidation, stress resistance and apoptosis, and mitochondrial fission. These findings confirmed that mitochondrion was one of the key targets of Cd toxicity. Moreover, dynamical regulations, such as reconstruction of energy homeostasis, induction of stress resistance and apoptosis, and morphological alterations, in mitochondria might play essential roles in Cd tolerance. Overall, this work provided a deep insight into the mitochondrial toxicity of Cd in clams based on a global mitochondrial proteomic analysis. (C) 2019 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41676114]; Young Taishan Scholars Program of Shandong Province [tsqn201812115]; Qingdao National Laboratory for Marine Science and Technology [QNLM201701]; Youth Innovation Promotion Association CAS [2015169]
收录类别SCI
语种英语
关键词[WOS]MUSSELS MYTILUS-GALLOPROVINCIALIS; VIRGINICA GMELIN BIVALVIA; SHRIMP CRANGON-AFFINIS; METABOLIC-RESPONSES; OXIDATIVE STRESS; METAL POLLUTION; MEMBRANE; INSIGHTS; FISSION; BOHAI
研究领域[WOS]Environmental Sciences
WOS记录号WOS:000474329700088
引用统计被引频次：17[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24896
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位1.Chinese Acad Sci, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai Inst Coastal Zone Res YIC, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Shandong, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Ji, CL,Lu, Z,Xu, LL,et al. Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics[J]. ENVIRONMENTAL POLLUTION,2019,251:802-810.
APAJi, CL.,Lu, Z.,Xu, LL.,Li, F.,Cong, M.,...&Wu, HF.(2019).Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics.ENVIRONMENTAL POLLUTION,251,802-810.
MLAJi, CL,et al."Evaluation of mitochondrial toxicity of cadmium in clam Ruditapes philippinarum using iTRAQ-based proteomics".ENVIRONMENTAL POLLUTION 251(2019):802-810.
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