Fu, XL; Wen, JH; Li, JW; Lin, H; Liu, YM; Zhuang, XM; Tian, CY; Chen, LX
发表期刊NANOSCALE
ISSN2040-3364
2019-09-07
卷号11期号:33页码:15530-15536
关键词POLYMERASE-CHAIN-REACTIONGOLD NANOPARTICLESSTRIP BIOSENSORPCRAMPLIFICATIONQUANTIFICATIONIMMUNOASSAYSUBSTRATESTRATEGYBINDING
研究领域Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied
DOI10.1039/c9nr04864b
产权排序[Fu, Xiuli; Wen, Jiahui; Li, Jingwen; Lin, Hao; Liu, Yongming; Zhuang, Xuming; Tian, Chunyuan] Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China; [Chen, Lingxin] Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
通讯作者Liu, Yongming(liuyongming100@126.com); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Accurate analysis of prostate cancer specific biomarkers plays an important role in the early diagnosis of prostate cancer. Traditional colorimetric lateral flow assay (LFA) has the limitations of low detection sensitivity and qualitative or semiquantitative detection. In this study, we developed a novel surface-enhanced Raman scattering (SERS)-based competitive LFA for the rapid and highly sensitive quantitative evaluation of prostate cancer antigen 3 (PCA3) mimic DNA. Herein, the competitive hybridization interaction with capture DNA between target PCA3 mimic DNA and reporter DNA-labeled SERS nanotags results in a change in the amount of SERS nanotags on the test line. The quantitative analysis of target PCA3 mimic DNA was realized by monitoring the Raman peak intensity of SERS nanotags on the test line. The limit of detection of PCA3 mimic DNA was estimated to be 3 fM, which is about three orders of magnitude more sensitive than that of a commercially available kit. By combining the outstanding characteristics of the well-established SERS-based competitive strategy and LFA platform, our design has strong potential for the early diagnosis of prostate cancer and other diseases.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21705139, 21575159, 21778047]; National Natural Science Foundation of Shandong Province in China [ZR2017BB026]
收录类别SCI
语种英语
关键词[WOS]POLYMERASE-CHAIN-REACTION; GOLD NANOPARTICLES; STRIP BIOSENSOR; PCR; AMPLIFICATION; QUANTIFICATION; IMMUNOASSAY; SUBSTRATE; STRATEGY; BINDING
研究领域[WOS]Chemistry, Multidisciplinary; Nanoscience & Nanotechnology; Materials Science, Multidisciplinary; Physics, Applied
WOS记录号WOS:000484345500008
引用统计被引频次：45[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24860
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Yantai Univ, Sch Chem & Chem Engn, Yantai 264005, Peoples R China;
2.Chinese Acad Sci, Res Ctr Coastal Environm Engn & Technol, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Pocesses & Ecol Reme, Yantai 264003, Peoples R China;
3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao 266237, Shandong, Peoples R China;
4.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Fu, XL,Wen, JH,Li, JW,et al. Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay[J]. NANOSCALE,2019,11(33):15530-15536.
APAFu, XL.,Wen, JH.,Li, JW.,Lin, H.,Liu, YM.,...&Chen, LX.(2019).Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay.NANOSCALE,11(33),15530-15536.
MLAFu, XL,et al."Highly sensitive detection of prostate cancer specific PCA3 mimic DNA using SERS-based competitive lateral flow assay".NANOSCALE 11.33(2019):15530-15536.
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