Qi, J; Li, BW
; Zhou, N; Wang, XY; Deng, DM; Luo, LQ; Chen, LX发表期刊BIOSENSORS & BIOELECTRONICS
ISSN0956-5663
2019-10-01
卷号142页码:UNSP 111533
关键词Paper based microfluidic deviceBioinspired molecularly imprinted techniqueElectrochemical detectionValvePoint of care diagnosisCancer markers
研究领域Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology
DOI10.1016/j.bios.2019.111533
产权排序[Qi, Ji; Deng, Dongmei; Luo, Liqiang] Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China; [Qi, Ji; Li, Bowei; Zhou, Na; Wang, Xiaoyan; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
通讯作者Li, Bowei(bwli@yic.ac.cn); Luo, Liqiang(luck@shu.edu.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要In this work, we provided a novel strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers (MIPs) on movable valve microfluidic paper-based electrochemical device (Bio-MIP-ePADs) for clinical detection of biomarkers. The newly movable valves on the device enable continuous and convenient delivery of fluid, which guarantee the performance for fabricating MIPs structure during long time electro-polymerization. Moreover, this strategy can directly detect antigens by taking advantage of molecular imprinting on paper-based device, which greatly decreases the cost during clinical testing, reduces the tedious washing procedure and does not need to consider the preservation of the antibody in enzyme linked immunosorbent assay (ELISA). This feature makes the chip suitable for the on-site family treatment or commercial products. To further validate the applicability of this proposed method for clinical diagnostic testing, carcinoembryonic antigen (CEA) was applied as prototyping model target for the clinical analysis. The proposed Bio-MIP-ePADs were cheap, easy to prepare, disposable and provided reliable analysis by comparing with ELISA. We hope the application of this technology will open up a new avenue to the point-of-care testing (POCT).
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41776110, 61571278, 61571280, 21804010]; National Key Research and Development Program of China [2016YFC1400702]; Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education MinistryScientific Research Foundation for the Returned Overseas Chinese Scholars
收录类别SCI
语种英语
关键词[WOS]ELECTROCHEMILUMINESCENCE; PLATFORM; MICROFLUIDICS; CHALLENGES; SENSORS; ASSAY
研究领域[WOS]Biophysics; Biotechnology & Applied Microbiology; Chemistry, Analytical; Electrochemistry; Nanoscience & Nanotechnology
WOS记录号WOS:000487175000057
引用统计被引频次：69[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24849
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Shanghai Univ, Coll Sci, Shanghai 200444, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China;
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Qi, J,Li, BW,Zhou, N,et al. The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device[J]. BIOSENSORS & BIOELECTRONICS,2019,142:UNSP 111533.
APAQi, J.,Li, BW.,Zhou, N.,Wang, XY.,Deng, DM.,...&Chen, LX.(2019).The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device.BIOSENSORS & BIOELECTRONICS,142,UNSP 111533.
MLAQi, J,et al."The strategy of antibody-free biomarker analysis by in-situ synthesized molecularly imprinted polymers on movable valve paper-based device".BIOSENSORS & BIOELECTRONICS 142(2019):UNSP 111533.
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