Zhang, JN; Zhang, YF; Chen, LZ; Yang, JM; Wei, QY; Yang, BW; Liu, XL; Yang, DL
发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2019-11
卷号94页码:230-238
关键词Venerupis philippinarumc-type lectinPattern recognition receptorImmune recognition
研究领域Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
DOI10.1016/j.fsi.2019.09.009
产权排序[Zhang, Jianning; Zhang, Yifei; Yang, Jianmin; Yang, Bowen; Liu, Xiaoli] Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China; [Wei, Qianyu; Yang, Dinglong] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Yang, Dinglong] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China; [Chen, Lizhu] Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
通讯作者Liu, Xiaoli(lxlshz2006@163.com); Yang, Dinglong(dlyang@yic.ac.cn)
作者部门中国科学院牟平海岸带环境综合试验站
英文摘要In the study, two c type lectins were identified and characterized from the manila clam Venerupis philippinarum (designed as VpClec-1 and VpClec-2, respectively). Multiple alignments and phylogenetic analysis strongly suggested that they were new members of the c-type lectin superfamily. In normal tissue of clams, both VpClec-1 and VpClec-2 transcripts were highly expressed in the tissue of hepatopancreas. After Vibrio anguillartun challenge, the temporal expression of both VpClec-1 and VpClec-2 transcripts was up-regulated in the hemocytes of manila clams. The recombinant protein VpClec-1 (rVpClec-1) showed obvious binding activities to lipopolysaccharide (LPS), peptidoglycan (PGN), glucan and zymosan in vitro, while the recombinant protein VpClec-2 (rVpClec-2) could only bind LPS, glucan and zymosan. Coinciding with the PAMPs binding assay, both rVpClec-1 and rVpClec-2 displayed broad agglutination and antibacterial activities towards Vibrio harveyi, Vibrio splendidus, Vibrio arquillarum, Enterobacter cloacae and Aeromonas hydrophila. Moreover, the phagocytosis and encapsulation ability of hemocytes could be significantly enhanced by rVpClec-1 and rVpClec-2. Notably, the rVpClec-1 but not rVpClec-2 elicited a chemotactic response from hemocytes. All the results showed that VpClec-1 and VpClec-2 functioned as pattern recognition receptors (PRRs) with distinct recognition spectrum, and involved in the innate immune responses of manila clams.
文章类型Article
资助机构Strategic Priority Research Program of the Chinese Academy of SciencesChinese Academy of Sciences [XDA23050303]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [41806196, 41506190]; Natural Science Foundation of Shandong ProvinceNatural Science Foundation of Shandong Province [ZR2019BD022]; 13th Five-year Informatization Plan of Chinese Academy of Sciences [XXH13506]
收录类别SCI
语种英语
关键词[WOS]ACID-BINDING LECTIN; MOLECULAR-CLONING; LITOPENAEUS-VANNAMEI; MANDUCA-SEXTA; PROTEIN; GENE; DOMAIN; PEPTIDOGLYCAN; IMMULECTIN-2; ACTIVATION
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000496892400025
引用统计被引频次：5[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24822
专题支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.Ludong Univ, Sch Life Sci, Yantai 264025, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Moping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China;
4.Shandong Marine Resource & Environm Res Inst, Yantai 264006, Peoples R China
推荐引用方式
GB/T 7714Zhang, JN,Zhang, YF,Chen, LZ,et al. Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization[J]. FISH & SHELLFISH IMMUNOLOGY,2019,94:230-238.
APAZhang, JN.,Zhang, YF.,Chen, LZ.,Yang, JM.,Wei, QY.,...&Yang, DL.(2019).Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization.FISH & SHELLFISH IMMUNOLOGY,94,230-238.
MLAZhang, JN,et al."Two c-type lectins from Venerupis philippinarum: Possible roles in immune recognition and opsonization".FISH & SHELLFISH IMMUNOLOGY 94(2019):230-238.
PDF全文下载地址:
点我下载PDF
