Huang, Y; He, N; Kang, Q; Shen, DZ; Wang, XY; Wang, YQ
; Chen, LX发表期刊ANALYST
ISSN0003-2654
2019-11-21
卷号144期号:22页码:6609-6616
关键词LABEL-FREE DETECTIONQUANTUM DOTSVITAMIN-CPROBESBIOTHIOLSNANODOTSFACILECANCERVITROCU2+
研究领域Chemistry, Analytical
DOI10.1039/c9an01694e
产权排序[Huang, Yan; Kang, Qi; Shen, Dazhong] Shandong Normal Univ, Minist Educ, Key Lab Mol & Nano Probes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China; [Huang, Yan; He, Na; Wang, Xiaoyan; Wang, Yunqing; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [He, Na; Wang, Xiaoyan; Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
通讯作者Kang, Qi(kangqi@sdnu.edu.cn); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Maintaining the redox balance of biological systems is a key point to maintain a healthy physiological environment. Excessive iron ions (Fe3+) can cause apoptosis, tissue damage and death. Fortunately, ascorbic acid (AA) as a reducing agent has been evaluated for the reduction of Fe3+. Moreover, AA plays an important role in relieving hypoxia-induced oxidative stress. Therefore, the real-time imaging of the Fe3+ and AA fluctuations is important for understanding their biofunctions in cells and in vivo. In this work, we developed a fluorescent nanoprobe carbon dot-desferrioxamine B (CD-DB) by the conjugate connection of CDs and desferrioxamine B (a complexing agent for Fe3+) for the associated detection of Fe3+ and AA. CD-DB exhibited excellent sensitivity and selectivity for the detection of Fe3+ and AA. The nanoprobe CDs-DB@Fe obtained by the reaction of CD-DB and Fe3+ was suitable for tracing the dynamic changes of AA in cells and in vivo. Therefore, CDs-DB@Fe was used for monitoring the fluctuation of AA in hypoxic cell models, hypoxic zebrafish models and liver ischemia mice models. These results exhibited the decrease in AA under hypoxic conditions because AA was consumed to neutralize free radicals and relieve hypoxia-induced oxidative stress damage. The ideal biocompatibility and low toxicity make our nanoprobe a potential candidate for the research of the physiological effects of AA in vivo.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21575159, 81573393, 21575080, 21976209]; program of Youth Innovation Promotion Association, CAS [2017256]; Instrument Developing Project of the Chinese Academy of Sciences [YZ201662]
收录类别SCI
语种英语
关键词[WOS]LABEL-FREE DETECTION; QUANTUM DOTS; VITAMIN-C; PROBES; BIOTHIOLS; NANODOTS; FACILE; CANCER; VITRO; CU2+
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000498842700011
引用统计被引频次：8[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24812
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Shandong Normal Univ, Minist Educ, Key Lab Mol & Nano Probes, Coll Chem Chem Engn & Mat Sci, Jinan 250014, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
4.Qufu Normal Univ, Coll Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式
GB/T 7714Huang, Y,He, N,Kang, Q,et al. A carbon dot-based fluorescent nanoprobe for the associated detection of iron ions and the determination of the fluctuation of ascorbic acid induced by hypoxia in cells and in vivo[J]. ANALYST,2019,144(22):6609-6616.
APAHuang, Y.,He, N.,Kang, Q.,Shen, DZ.,Wang, XY.,...&Chen, LX.(2019).A carbon dot-based fluorescent nanoprobe for the associated detection of iron ions and the determination of the fluctuation of ascorbic acid induced by hypoxia in cells and in vivo.ANALYST,144(22),6609-6616.
MLAHuang, Y,et al."A carbon dot-based fluorescent nanoprobe for the associated detection of iron ions and the determination of the fluctuation of ascorbic acid induced by hypoxia in cells and in vivo".ANALYST 144.22(2019):6609-6616.
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