Yang, Q; Wang, XY; Peng, HL; Arabi, M; Li, JH
; Xiong, H; Choo, J; Chen, LX发表期刊SENSORS AND ACTUATORS B-CHEMICAL
2020
卷号302页码:127176
关键词Alkaline phosphataseVisual detectionRatiometric fluorescenceColorimetryDual-mode assay
研究领域Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
DOI10.1016/j.snb.2019.127176
产权排序[Yang, Qian; Peng, Hailong; Xiong, Hua] Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China; [Yang, Qian; Wang, Xiaoyan; Arabi, Maryam; Li, Jinhua; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wang, Xiaoyan] Binshou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
通讯作者Xiong, Hua(huaxiong100@126.com); Choo, Jaebum(jbchoo@cau.ac.kr); Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Alkaline phosphatase (ALP) activity plays a crucial role in foods and varies greatly in livestock and dairy products; it is quite difficult to compatibly monitor the different activities in various complicated foods. Herein, we proposed a ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide (MnO2) nanosheets for the visualization of ALP activity in livestock serum and pasteurized milk samples compatibly. MnO2 nanosheets could oxidize dopamine into green fluorescent polydopamine (PDA) nanoparticles, so to quench the red fluorescent quantum dots (QDs), or oxidize colorless 3,3',5,5'-tetramethylbenzidine (TMB) into yellow TMBox. In the coexistence of ALP and 2-phospho-L-ascorbic acid, MnO2 nanosheets were reduced into Mn2+ ions by the catalysate of ascorbic acid, failing to generate PDA nanoparticles, and therefore, recovering the fluorescence of QDs for ratiometric fluorescence detection of high-activity ALP, or weakening the oxidization of TMB for colorimetric detection of ultralow-activity ALP. The ratiometric fluorescence-colorimetry combination extended the linear range over three orders of magnitude (0.04-80 mU/mL), and lowered the detection limit down to 0.015 mU/mL, along with profuse ALP-dependent (fluorescence) color changes for visual detection of ALP activity. Excellent recognition selectivity for ALP was attained over possibly coexistent reducing substances. Furthermore, the endogenous ALP were detected ranging from 17.32 to 269.54 mU/mL in seven typical livestock sera, consistent with that measured by commercial ALP assay kit; the detection results for ALP in four pasteurized milks matched well with that by test paper. The developed dual-mode assay held great potential for rapid on-site visual determination of reductant-related analytes in complicated matrices.
文章类型Article
资助机构National Natural Science Foundation of ChinaNational Natural Science Foundation of China [21667018, 31860470, 21876199, 21804010, 21976209, 31660482, 41776110]; Chinese Academy of Sciences President's International Fellowship Initiative [2019PC0050]; National Research Foundation of KoreaNational Research Foundation of Korea [2019R1A2C3004375, 2017M3D1A1039287, 2018M3A7B4071203]
收录类别SCI
语种英语
关键词[WOS]POLYDOPAMINE NANOPARTICLES; MNO2 NANOSHEETS; GLUTATHIONE DETECTION; SENSING PLATFORM; CARBON DOTS; TURN-OFF; IMMUNOASSAY
研究领域[WOS]Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation
WOS记录号WOS:000489830200033
引用统计被引频次：40[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24785
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Nanchang Univ, State Key Lab Food Sci & Technol, Nanchang 330047, Jiangxi, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Res Ctr Coastal Environm Engn & Technol, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
3.Binshou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
4.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea;
5.Chinese Acad Sci, Ctr Ocean Megasci, Qingdao 266071, Shandong, Peoples R China
推荐引用方式
GB/T 7714Yang, Q,Wang, XY,Peng, HL,et al. Ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide nanosheets for visual detection of alkaline phosphatase activity[J]. SENSORS AND ACTUATORS B-CHEMICAL,2020,302:127176.
APAYang, Q.,Wang, XY.,Peng, HL.,Arabi, M.,Li, JH.,...&Chen, LX.(2020).Ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide nanosheets for visual detection of alkaline phosphatase activity.SENSORS AND ACTUATORS B-CHEMICAL,302,127176.
MLAYang, Q,et al."Ratiometric fluorescence and colorimetry dual-mode assay based on manganese dioxide nanosheets for visual detection of alkaline phosphatase activity".SENSORS AND ACTUATORS B-CHEMICAL 302(2020):127176.
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