Wang, Y
; Zhang, LW; Chen, LX发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2020-01-21
卷号92期号:2页码:1997-2004
关键词IN-VIVOSELENOLSELENOCYSTEINESELENOPROTEINNEUROTOXICITYMETABOLISMSPECIATIONREDUCTASEPROTEINSSTRESS
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.9b04381
产权排序[Wang, Yue; Zhang, Liangwei; Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China; [Chen, Lingxin] Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China; [Zhang, Liangwei; Chen, Lingxin] Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China; [Wang, Yue] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Chen, Lingxin] Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
通讯作者Chen, Lingxin(lxchen@yic.ac.cn)
作者部门海岸带环境工程技术研究与发展中心
英文摘要Solid evidence confirms that glutathione peroxidase (GPx) is a kind of vital protease in the first-line antioxidant defense system and participates in regulation of redox homeostasis as well as the pentose phosphate pathway. However, the current methods cannot achieve real-time and in situ visualization studies of GPx. In addition, GPx is highly reactive and susceptible to external interference, and there is rare research for exploring the roles of GPx under environmental factor exposure. Herein, we report a novel two-photon ratiometric fluorescent probe (TP-SS) for GPx detection for the first time. Using TP-SS, we explore the reversible catalytic cycle and the antioxidant mechanisms of GPx/GSH redox pool in aging and mercury exposure models. We detect the concentration fluctuation of GPx in aging and mercury exposure mice models. Also, we perform GPx detection in deep brain tissue and the imaging depth up to 100 mu m. We believe that the novel two-photon ratiometric fluorescent probe TP-SS can facilitate the development of GPx-targeting tools and offer great advances in exploring the physiological/pathological functions of GPx.
文章类型Article
资助机构National Nature Science Foundation of ChinaNational Natural Science Foundation of China [21976209, 21778026, 21575159]; program of Youth Innovation Promotion Association, CAS [2019217]; Taishan Scholar Project Special Funding [ts20190962]
收录类别SCI
语种英语
关键词[WOS]IN-VIVO; SELENOL; SELENOCYSTEINE; SELENOPROTEIN; NEUROTOXICITY; METABOLISM; SPECIATION; REDUCTASE; PROTEINS; STRESS
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000509420400047
引用统计被引频次：21[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24765
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Res Ctr Coastal Environm Engn & Technol, Yantai 264003, Peoples R China;
2.Binzhou Med Univ, Sch Pharm, Yantai 264003, Peoples R China;
3.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao 266071, Peoples R China;
4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
5.Qufu Normal Univ, Dept Chem & Chem Engn, Qufu 273165, Shandong, Peoples R China
推荐引用方式
GB/T 7714Wang, Y,Zhang, LW,Chen, LX. Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models[J]. ANALYTICAL CHEMISTRY,2020,92(2):1997-2004.
APAWang, Y,Zhang, LW,&Chen, LX.(2020).Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models.ANALYTICAL CHEMISTRY,92(2),1997-2004.
MLAWang, Y,et al."Glutathione Peroxidase-Activatable Two-Photon Ratiometric Fluorescent Probe for Redox Mechanism Research in Aging and Mercury Exposure Mice Models".ANALYTICAL CHEMISTRY 92.2(2020):1997-2004.
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