Lu, Z; Wang, S; Ji, CL
; Li, F; Cong, M; Shan, XJ; Wu, HF发表期刊ENVIRONMENTAL POLLUTION
ISSN0269-7491
2020-02
卷号257页码:113591
关键词CadmiumParalichthys olivaceusiTRAQProteomicsMitochondrial toxicity
研究领域Environmental Sciences
DOI10.1016/j.envpol.2019.113591
产权排序[Lu, Zhen; Wang, Shuang; Ji, Chenglong; Li, Fei; Cong, Ming; Wu, Huifeng] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China; [Ji, Chenglong; Shan, Xiujuan] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China; [Lu, Zhen; Wang, Shuang] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
通讯作者Wu, Huifeng(hfwu@yic.ac.cn)
作者部门海岸带环境过程实验室
英文摘要Cadmium (Cd) is an important heavy metal pollutant in the Bohai Sea. Mitochondria are recognized as the key target for Cd toxicity. However, mitochondria! responses to Cd have not been fully investigated in marine fishes. In this study, the mitochondrial responses were characterized in gills of juvenile flounder Paralichthys olivaceus treated with two environmentally relevant concentrations (5 and 50 mu g/L) of Cd for 14 days by determination of mitochondrial membrane potential (MMP), observation of mitochondrial morphology and quantitative proteomic analysis. Both Cd treatments significantly decreased MMPs of mitochondria from flounder gills. Mitochondrial morphologies were altered in Cd-treated flounder samples, indicated by more and smaller mitochondria. iTRAQ-based proteomic analysis indicated that a total of 128 proteins were differentially expressed in both Cd treatments. These proteins were basically involved in various biological processes in gill mitochondria, including mitochondrial morphology and import, tricarboxylic acid (TCA) cycle, oxidative phosphorylation (OXPHOS), primary bile acid biosynthesis, stress resistance and apoptosis. These results indicated that dynamic regulations of energy homeostasis, cholesterol metabolism, stress resistance, apoptosis, and mitochondrial morphology in gill mitochondria might play significant roles in response to Cd toxicity. Overall, this study provided a global view on mitochondrial toxicity of Cd in flounder gills using iTRAQ-based proteomics. (C) 2019 Elsevier Ltd. All rights reserved.
文章类型Article
资助机构NSFCNational Natural Science Foundation of China [41676114]; Young Taishan Scholars Program of Shandong Province [tsqn201812115]; Qingdao National Laboratory for Marine Science and Technology [QNLM201701]
收录类别SCI
语种英语
关键词[WOS]VIRGINICA GMELIN BIVALVIA; BILE-ACID SYNTHESIS; ENERGY-METABOLISM; ROS PRODUCTION; COMPLEX-II; PROTEIN; MITOFILIN; ACCUMULATION; TARGET; SAM50
研究领域[WOS]Environmental Sciences
WOS记录号WOS:000514746800091
引用统计被引频次：11[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24756
专题中科院海岸带环境过程与生态修复重点实验室
中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, CAS Key Lab Coastal Environm Proc & Ecol Remediat, Shandong Key Lab Coastal Environm Proc,YICCAS, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Fisheries Sci & Food Prod Proc, Qingdao 266237, Peoples R China;
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Lu, Z,Wang, S,Ji, CL,et al. iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium[J]. ENVIRONMENTAL POLLUTION,2020,257:113591.
APALu, Z.,Wang, S.,Ji, CL.,Li, F.,Cong, M.,...&Wu, HF.(2020).iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium.ENVIRONMENTAL POLLUTION,257,113591.
MLALu, Z,et al."iTRAQ-based proteomic analysis on the mitochondrial responses in gill tissues of juvenile olive flounder Paralichthys olivaceus exposed to cadmium".ENVIRONMENTAL POLLUTION 257(2020):113591.
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