Wu, Y; Choi, N; Chen, H; Dang, H; Chen, LX
; Choo, J发表期刊ANALYTICAL CHEMISTRY
ISSN0003-2700
2020-02-04
卷号92期号:3页码:2628-2634
关键词MULTIPLEX DETECTIONAMPLIFICATION-FREESERSPCRDNASPECTROSCOPYIMMUNOASSAYMUTATIONSDIAGNOSIS
研究领域Chemistry, Analytical
DOI10.1021/acs.analchem.9b04522
产权排序[Chen, Lingxin] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China; [Wu, Yixuan; Chen, Hao; Dang, Hajun; Choo, Jaebum] Chung Ang Univ, Dept Chem, Seoul 06974, South Korea; [Choi, Namhyun] Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea
通讯作者Chen, Lingxin(lxchen@yic.ac.cn); Choo, Jaebum(jbchoo@cau.ac.kr)
作者部门海岸带环境工程技术研究与发展中心
英文摘要We report a surface-enhanced Raman scattering (SERS)-based polymerase chain reaction (PCR) assay platform for the sensitive and rapid detection of a DNA marker (pagA) of Bacillus anthracis. Real-time quantitative PCR (RT-qPCR) has been recently considered a gold standard for the quantitative evaluation of a target gene, but it still suffers from the problem of a long thermocycling time. To address this issue, we developed a conceptually new SERS-PCR platform and evaluated its performance by sequentially measuring the Raman signals of B. anthracis DNA after the completion of different thermocycling numbers. According to our experimental data, SERS-PCR has lower limits of detection (LODs) than RT-qPCR under the small cycle number of 20. Particularly, it was impossible to detect a target DNA amplicon using RT-qPCR before the number of cycles reached 15, but SERS-PCR enabled DNA detection after only five cycles with an LOD value of 960 pM. In addition, the dynamic range for SERS-PCR (0.1-1000 pM) is wider than that for RTqPCR (150-1000 pM) under the same condition. We believe that this SERS-PCR technique has a strong potential to be a powerful tool for the rapid and sensitive diagnosis of infectious diseases in the near future.
文章类型Article
资助机构National Research Foundation of KoreaNational Research Foundation of Korea [2019R1A2C3004375]; Chung-Ang University
收录类别SCI
语种英语
关键词[WOS]MULTIPLEX DETECTION; AMPLIFICATION-FREE; SERS; PCR; DNA; SPECTROSCOPY; IMMUNOASSAY; MUTATIONS; DIAGNOSIS
研究领域[WOS]Chemistry, Analytical
WOS记录号WOS:000511509400038
引用统计被引频次：15[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24755
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境工程技术研究与发展中心
中科院海岸带环境过程与生态修复重点实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Environm Proc & Ecol Remediat, Yantai 264003, Peoples R China;
2.Chung Ang Univ, Dept Chem, Seoul 06974, South Korea;
3.Hanyang Univ, Dept Bionano Technol, Ansan 15588, South Korea
推荐引用方式
GB/T 7714Wu, Y,Choi, N,Chen, H,et al. Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays[J]. ANALYTICAL CHEMISTRY,2020,92(3):2628-2634.
APAWu, Y,Choi, N,Chen, H,Dang, H,Chen, LX,&Choo, J.(2020).Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays.ANALYTICAL CHEMISTRY,92(3),2628-2634.
MLAWu, Y,et al."Performance Evaluation of Surface-Enhanced Raman Scattering-Polymerase Chain Reaction Sensors for Future Use in Sensitive Genetic Assays".ANALYTICAL CHEMISTRY 92.3(2020):2628-2634.
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