Zhang, XH; Lin, SJ; Liu, DY
发表期刊ENVIRONMENTAL MICROBIOLOGY
ISSN1462-2912
2020-05
卷号22期号:5页码:9
关键词DISSOLVED ORGANIC PHOSPHORUSALKALINE-PHOSPHATASE ACTIVITYRNA-SEQ DATAEXTRACELLULAR NUCLEOTIDESNITROGEN-FIXATIONMARINEPHYTOPLANKTONLIMITATIONOCEANCYANOBACTERIUM
研究领域Microbiology
DOI10.1111/1462-2920.14944
产权排序[Zhang, Xiaohua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China; [Zhang, Xiaohua] Univ Chinese Acad Sci, Beijing 100049, Peoples R China; [Zhang, Xiaohua] Binzhou Med Univ, Dept Gene Engn, Yantai 264003, Peoples R China; [Lin, Senjie] Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA; [Liu, Dongyan] East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China
通讯作者Liu, Dongyan(dyliu@sklec.ecnu.edu.cn)
作者部门海岸带环境过程实验室
英文摘要The capacity of phytoplankton to utilize dissolved organic phosphorus (DOP) plays an important role in their competition for resources when the availability of dissolved inorganic phosphorus (DIP) is low in the aquatic systems. Here, we explored the physiological and molecular responses of a globally distributed marine diatom, Skeletonema costatum, in utilizing adenosine-5 '-triphosphate (ATP) based on incubation experiments under ATP, DIP-replete, and DIP-depleted conditions. The results show that ATP supports the growth of S. costatum as efficiently as DIP. The pathway of S. costatum involved in utilizing ATP is not related to alkaline phosphatase (AP), an important DOP hydrolase, although extracellular hydrolysis is involved. The transcriptome analysis revealed several transcripts related to the hydrolase activity (e.g. NAD+ diphosphatase), which were significantly upregulated in the ATP culture group, indicating their possible involvement in ATP hydrolysis. Meanwhile, ATP-grown S. costatum exhibited downregulation of the genes related to a series of metabolic activities (e.g. purine metabolism), apparently to adapt to ATP condition.
文章类型Article
资助机构Natural Science Foundation of ChinaNational Natural Science Foundation of China [41876127, 41776116]; Ministry of Science and Technology, ChinaMinistry of Science and Technology, China [2016YFE0133700, 2016YFA0600904]
收录类别SCI
语种英语
关键词[WOS]DISSOLVED ORGANIC PHOSPHORUS; ALKALINE-PHOSPHATASE ACTIVITY; RNA-SEQ DATA; EXTRACELLULAR NUCLEOTIDES; NITROGEN-FIXATION; MARINE; PHYTOPLANKTON; LIMITATION; OCEAN; CYANOBACTERIUM
研究领域[WOS]Microbiology
WOS记录号WOS:000514890900001
引用统计被引频次：2[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24747
专题中科院海岸带环境过程与生态修复重点实验室_海岸带环境过程实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Zone Environm Proc & Ecol Remedia, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China;
3.Binzhou Med Univ, Dept Gene Engn, Yantai 264003, Peoples R China;
4.Univ Connecticut, Dept Marine Sci, Groton, CT 06340 USA;
5.East China Normal Univ, State Key Lab Estuarine & Coastal Res, Shanghai 200241, Peoples R China
推荐引用方式
GB/T 7714Zhang, XH,Lin, SJ,Liu, DY. Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization[J]. ENVIRONMENTAL MICROBIOLOGY,2020,22(5):9.
APAZhang, XH,Lin, SJ,&Liu, DY.(2020).Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization.ENVIRONMENTAL MICROBIOLOGY,22(5),9.
MLAZhang, XH,et al."Transcriptomic and physiological responses of Skeletonema costatum to ATP utilization".ENVIRONMENTAL MICROBIOLOGY 22.5(2020):9.
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