其他题名Response of serine/threonine protein kinase SpkC to high temperature stress in Synechocystis sp. PCC 6803
赵佳琳; 陈军; 崔玉琳
; 于淑贤; 陈高; 秦松发表期刊微生物学报
ISSN0001-6209
2018-03-07
卷号58期号:10页码:1732-1742
关键词集胞藻PCC 6803丝氨酸/苏氨酸激酶SpkC高温胁迫
研究领域植物学
DOI10.13343/j.cnki.wsxb.20170546
产权排序(1)中国科学院烟台海岸带研究所; (2)中国科学院大学; (3)山东省农业科学院生物技术研究中心; (4)山东省作物遗传改良与生态生理重点实验室
作者部门海岸带生物学与生物资源保护实验室
英文摘要Serine/threonine protein kinases play important roles to sense and transduce signal to cope with external environment. However, functions of many serine/threonine protein kinases in cyanobacteria are still unknown. [Objective] This study aims to explore whether SpkC in Synechocystis sp. PCC 6803 responses to high temperature stress. [Methods] We used the homologous recombination method to construct the spkC gene knockout mutant (ΔspkC), and then compared the growth rate, pigment contents of ΔspkC mutant and the wild strain under high temperature stress. In addition, we detected chlorophyll fluorescence under high temperature stress. Recoveries of ΔspkC and wild strain from high-temperature stress were determined through measuring the growth rate under normal temperature. [Results] Compared with wild type strain, ΔspkC mutant grew slower, and the contents of three photosynthetic pigments (chlorophyll, carotenoid and phycobilin) decreased after high temperature stress at 42 °C. The activity of photosynthetic system II in ΔspkC decreased more rapidly at 45 °C. What's more, ΔspkC strain was not able to recover after high-temperature stress for 5 days; their survival rate was significantly lower than wild-type. [Conclusion] The deletion of spkC led to severe defect response to high-temperature stress in Synechocystis sp. PCC 6803, which suggests that serine/threonine protein kinase SpkC is involved in response processes against high temperature stress.
中文摘要丝氨酸/苏氨酸激酶是蓝藻感知和转导外界刺激的重要元件,但至今蓝藻中很多丝氨酸/苏氨酸激酶的功能尚属未知。【目的】研究集胞藻PCC6803中的丝氨酸/苏氨酸激酶Spk C是否参与对高温胁迫的响应。【方法】本研究采用同源重组的方法构建spC基因完全敲除突变株,检测突变株与野生株在高温胁迫下的生长状况、色素组成,并对高温胁迫下叶绿素荧光参数差异进行分析,比较光合系统Ⅱ活性差异。此外,通过测定生长速率来判断高温胁迫后藻株的恢复情况。【结果】经过42℃高温胁迫后,与野生株相比,突变株ΔspkC生长减缓,光合色素(叶绿素、类胡萝卜素和藻胆色素)的含量降低;45℃高温胁迫下突变株ΔspkC的光合系统Ⅱ活性下降幅度更大;经过5 d 42℃高温处理后,突变株生长几乎停滞,存活率较野生株明显降低。【结论】集胞藻PCC 6803中spkC基因的缺失导致突变株对高温胁迫响应出现缺陷,提示丝氨酸/苏氨酸激酶SpkC参与响应高温胁迫。
资助机构国家自然科学基金#41406192; 国家自然科学基金#41176144; 国家自然科学基金#41376139; 国家重点研发计划#2016YFF0202304; 烟台市科技计划#2016JHZB007
收录类别CSCD
语种中文
CSCD记录号CSCD:6332914
引用统计
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24722
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.中国科学院烟台海岸带研究所;
2.中国科学院大学;
3.山东省农业科学院生物技术研究中心;
4.山东省作物遗传改良与生态生理重点实验室
推荐引用方式
GB/T 7714赵佳琳,陈军,崔玉琳,等. 集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应[J]. 微生物学报,2018,58(10):1732-1742.
APA赵佳琳,陈军,崔玉琳,于淑贤,陈高,&秦松.(2018).集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应.微生物学报,58(10),1732-1742.
MLA赵佳琳,et al."集胞藻PCC 6803中丝氨酸/苏氨酸激酶SpkC对高温胁迫的响应".微生物学报 58.10(2018):1732-1742.
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