Min, Jun
; Chen, Weiwei; Hu, Xiaoke发表期刊JOURNAL OF HAZARDOUS MATERIALS
ISSN0304-3894
2019-01-05
卷号361页码:10-18
关键词2,6-Dibromo-4-nitrophenolBiodegradationCatabolic mechanismCupriavidus sp.FADH(2)-dependent monooxygenase
研究领域Engineering; Environmental Sciences & Ecology
DOI10.1016/j.jhazmat.2018.08.063
产权排序[Min, Jun; Chen, Weiwei; Hu, Xiaoke] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Min, Jun; Hu, Xiaoke] Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Compound 2,6-dibromo-4-nitrophenol (2,6-DBNP) with high cytotoxicity and genotoxicity has been recently identified as an emerging brominated disinfection by-product during chloramination and chlorination of water, and its environmental fate is of great concern. To date, the biodegradation process of 2,6-DBNP is unknown. Herein, Cupriavidus sp. strain CNP-8 was reported to be able to utilize 2,6-DBNP as a sole source of carbon, nitrogen and energy. It degraded 2,6-DBNP in concentrations up to 0.7 mM, and the degradation of 2,6-DBNP conformed to Haldane inhibition model with mu(max) of 0.096 h(-1), K-s of 0.05 mM and K-i of 0.31 mM. Comparative transcriptome and real-time quantitative PCR analyses suggested that the hnp gene cluster was likely responsible for 2,6-DBNP catabolism. Three Hnp proteins were purified and functionally verified. HnpA, a FADH(2)-dependent monooxygenase, was found to catalyze the sequential denitration and debromination of 2,6-DBNP to 6-bromohydroxyquinol (6-BHQ) in the presence of the flavin reductase HnpB. Gene knockout and complementation revealed that hnpA is essential for strain CNP-8 to utiluze 2,6-DBNP. HnpC, a 6-BHQ 1,2-dioxygenase was proposed to catalyze the ring-cleavage of 6-BHQ during 2,6-DBNP catabolism. These results fill a gap in the understanding of the microbial degradation process and mechanism of 2,6-DBNP.
文章类型Article
资助机构National Natural Science Foundation of China [31600085]; State's Key Project of Research and Development Plan [2016YFC1402300]; Foreword Key Priority Research Program of Chinese Academy of Sciences [QYZDB-SSW-DQC013]; State Key Laboratory of Microbial Metabolism, Shanghai JiaoTong University [MMLKF17-04]; Yantai Science and Technology Project [2017ZH092]
收录类别SCI
语种英语
关键词[WOS]DISINFECTION BY-PRODUCTS; P-NITROPHENOL; 2,4,6-TRICHLOROPHENOL DEGRADATION; CONTAMINATED SOIL; BIOAUGMENTATION; CATABOLISM; 2-CHLORO-4-NITROPHENOL; MONOOXYGENASE; EFFLUENTS; CLUSTER
研究领域[WOS]Engineering, Environmental; Environmental Sciences
WOS记录号WOS:000449125800002
引用统计被引频次：18[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24617
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China;
2.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714Min, Jun,Chen, Weiwei,Hu, Xiaoke. Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization[J]. JOURNAL OF HAZARDOUS MATERIALS,2019,361:10-18.
APAMin, Jun,Chen, Weiwei,&Hu, Xiaoke.(2019).Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization.JOURNAL OF HAZARDOUS MATERIALS,361,10-18.
MLAMin, Jun,et al."Biodegradation of 2,6-dibromo-4-nitrophenol by Cupriavidus sp strain CNP-8: Kinetics, pathway, genetic and biochemical characterization".JOURNAL OF HAZARDOUS MATERIALS 361(2019):10-18.
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