VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC
Zhou, Ling; Yi, Yuetao; Yuan, Qing; Zhang, Jing; Li, Youjie; Wang, Pingyu; Xu, Maolei; Xie, Shuyang
发表期刊FREE RADICAL BIOLOGY AND MEDICINE
ISSN0891-5849
2018-12
卷号129页码:177-185
关键词Vanadyl alginate oligosaccharidesNon-small cell lung cancerROSAKTPTEN
研究领域Biochemistry & Molecular Biology; Endocrinology & Metabolism
DOI10.1016/j.freeradbiomed.2018.09.016
产权排序[Zhou, Ling; Yuan, Qing; Zhang, Jing; Li, Youjie; Wang, Pingyu; Xie, Shuyang] Binzhou Med Univ, Dept Biochem & Mol Biol, Key Lab Tumor Mol Biol, Yantai 264003, Shandong, Peoples R China; [Zhou, Ling; Xu, Maolei] Binzhou Med Univ, Sch Pharm, Key Lab Tradit Chinese Med Prescript Effect & Cli, State Adm Tradit Chinese Med, Yantai 264003, Shandong, Peoples R China; [Yi, Yuetao] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Previous studies have confirmed that protein tyrosine phosphatase 1B (PTP1B) can promote tumour progression in non-small cell lung cancer (NSCLC). Vanadyl alginate oligosaccharides (VAOS) is a new coordination compounds that possesses a good PTP1B inhibitory activity. However, the potent anticancer efficacy of VAOS in human NSCLC requires further study. In this study, VAOS exhibited effective inhibitory effects in NSCLC both in cultured cells and in a xenograft mouse model. VAOS was further identified to induce NSCLC cell apoptosis through activating protein kinase B (AKT) to elevate intracellular reactive oxygen species (ROS) levels by increasing in oxygen consumption and impairing the ROS-scavenging system. Neither silencing of PTP1B by siRNA nor transient overexpression of PTP1B had an effect on the AKT phosphorylation triggered by VAOS, indicating that PTP1B inhibition was not involved in VAOS-induced apoptosis. Through phosphorus colorimetric assay, we demonstrated that VAOS notably inhibited phosphatase and tensin homologue deleted on chromosome 10 (PTEN) dephosphorylation activity, another member of the protein tyrosine phosphatases (PTPases)-upstream factor of AKT. Interestingly, PTEN knockdown sensitized cells to VAOS, whereas ectopic expression of PTEN markedly rescued VAOS-mediated lethality. In vivo, VAOS treatment markedly reduced PTEN activity and tumour cell burden with low systemic toxicity. Thus, our data not only provided a new therapeutic drug candidate for NSCLC, but presented new understanding into the pharmacological research of VAOS.
文章类型Article
资助机构National Natural Science Foundation of China [81772281]; Natural Science Foundation of Shandong Province [ZR2018BH046, ZR2018QH005]; Project of Shandong Province Higher Educational Science and Technology Program [J15LM51]; Taishan Scholar Project of Shandong Province [ts201712067]
收录类别SCI
语种英语
关键词[WOS]HEPATOCELLULAR-CARCINOMA; OXIDATIVE STRESS; PTEN; CANCER; CELLS; MODULATION; MECHANISMS; SENESCENCE
研究领域[WOS]Biochemistry & Molecular Biology; Endocrinology & Metabolism
WOS记录号WOS:000450298400016
引用统计被引频次：4[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24590
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室

作者单位1.Binzhou Med Univ, Dept Biochem & Mol Biol, Key Lab Tumor Mol Biol, Yantai 264003, Shandong, Peoples R China;
2.Binzhou Med Univ, Sch Pharm, Key Lab Tradit Chinese Med Prescript Effect & Cli, State Adm Tradit Chinese Med, Yantai 264003, Shandong, Peoples R China;
3.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264003, Peoples R China

推荐引用方式
GB/T 7714Zhou, Ling,Yi, Yuetao,Yuan, Qing,et al. VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC[J]. FREE RADICAL BIOLOGY AND MEDICINE,2018,129:177-185.
APAZhou, Ling.,Yi, Yuetao.,Yuan, Qing.,Zhang, Jing.,Li, Youjie.,...&Xie, Shuyang.(2018).VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC.FREE RADICAL BIOLOGY AND MEDICINE,129,177-185.
MLAZhou, Ling,et al."VAOS, a novel vanadyl complexes of alginate saccharides, inducing apoptosis via activation of AKT-dependent ROS production in NSCLC".FREE RADICAL BIOLOGY AND MEDICINE 129(2018):177-185.


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