Li, Jie
; Wang, Mingchao; Qiao, Yingyun; Tian, Yuanyu; Liu, Junhong; Qin, Song; Wu, Wenhui发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
2018-11
卷号74页码:156-163
关键词TilapiaCollagenFish skinMarine biomaterialsMicrofiber matrix scaffolds
研究领域Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
DOI10.1016/j.procbio.2018.07.009
产权排序[Li, Jie; Wang, Mingchao; Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264000, Peoples R China; [Qiao, Yingyun; Tian, Yuanyu] China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China; [Liu, Junhong] Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266000, Peoples R China; [Wu, Wenhui] Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Marine Pharmacol, Shanghai 201306, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Aquatic origin collagen (Aqua-collagen) has been pursued as an alternative to mammalian origins. Acid-soluble collagen (ASC) and pepsin-soluble collagen (PSC) were successfully extracted from the skin of tilapia with the yield about 19-20% (basis of lyophilized dry weight), and examined for their physico-functional and structural properties. Both ASC and PSC containing at, a2 chains were characterized to be type I collagen and had lower denaturation temperature compared to mammalian origins. PSC (low telopeptides collagen) was selected for biomedical scaffolds construction due to its low immunogenicity. SEM analysis of fish collagen scaffolds showed a wide range of pore size distribution, high porosity, and high surface area-to-volume ratios. The tilapia collagen microfiber matrix scaffolds were grafted beneath the dorsal skin in 96 mice towards tissue regeneration, with bovine collagen microfiber matrix scaffolds (Avitene" UltraFoamn" sponge) serving as control. Biocompatibility evaluation in the dorsal tissue showed that implanted scaffolds degraded completely after 20 days with no pathological inflammatory tissue responses. These findings indicated that aqua-collagens microfiber matrix scaffolds were highly biocompatible in nature, exploring its feasibility for the development of scaffolds in tissue engineering.
文章类型Article
资助机构key program of the Chinese Academy of Sciences [KFZD-SW-218]; Science and Technology Development Project of Shandong Province [2015GSF115032]; 2016 Strategic Emerging Industry Development Fund (Starting Fund for Marine Economy Development and Innovation Demonstration City) [YHCX-SW-Y-201701]
收录类别SCI
语种英语
关键词[WOS]PEPSIN-SOLUBLE COLLAGEN; EXTRACELLULAR-MATRIX; CONNECTIVE TISSUES; CROSS-LINKING; OUTER SKIN; BONE; ACID; BIOMATERIALS; SCALE; DENATURATION
研究领域[WOS]Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
WOS记录号WOS:000447577800020
引用统计被引频次：37[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24561
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Yantai 264000, Peoples R China;
2.China Univ Petr East China, State Key Lab Heavy Oil Proc, Qingdao 266580, Peoples R China;
3.Qingdao Univ Sci & Technol, Coll Chem Engn, Qingdao 266000, Peoples R China;
4.Shanghai Ocean Univ, Coll Food Sci & Technol, Dept Marine Pharmacol, Shanghai 201306, Peoples R China
推荐引用方式
GB/T 7714Li, Jie,Wang, Mingchao,Qiao, Yingyun,et al. Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering[J]. PROCESS BIOCHEMISTRY,2018,74:156-163.
APALi, Jie.,Wang, Mingchao.,Qiao, Yingyun.,Tian, Yuanyu.,Liu, Junhong.,...&Wu, Wenhui.(2018).Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering.PROCESS BIOCHEMISTRY,74,156-163.
MLALi, Jie,et al."Extraction and characterization of type I collagen from skin of tilapia (Oreochromis niloticus) and its potential application in biomedical scaffold material for tissue engineering".PROCESS BIOCHEMISTRY 74(2018):156-163.
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