Ge, Baosheng; Chen, Yao; Yu, Qian; Lin, Xiaojun; Li, Jiqiang; Qin, Song
发表期刊PROCESS BIOCHEMISTRY
ISSN1359-5113
2018-08
卷号71页码:23-30
关键词PhycocyanobilinHeme biosynthetic pathway5-Aminolevulinic acidRegulationCombinatorial biosynthesisEscherichia coli
研究领域Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering
DOI10.1016/j.procbio.2018.05.011
产权排序[Ge, Baosheng; Chen, Yao; Yu, Qian; Lin, Xiaojun; Li, Jiqiang] China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China; [Qin, Song] Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Due to its biochemical and spectroscopic properties, phycocyanobilin (PCB) is of special interest for a variety of applications, but the production of PCB from Spirulina is complicated and costly. Here we report the assembly and regulation of the entire heme biosynthetic pathway for combinatorial biosynthesis of PCB in Escherichia coli. Our results shows that a plasmid containing bicistronic expression of hoxi and pcyA yields 6.64 mg/L PCB, which is higher than that of plasmid with one promoter for both genes (p < 0.01). Up-regulation of hemB, hemG, hemH, hemA(s), and hemL individually or simultaneously does not significantly improve the yield of PCB, but induces accumulation of 5-Aminolevulinic acid (ALA) and heme. Increasing the copy number of hoxi and pcyA genes slightly improves the production of PCB by enhancing the expression level of Hox1 and PcyA enzymes, suggesting that this two-step enzymatic reaction is the rate-limiting step for the production of PCB. This work provides new important insights into the regulatory mechanism of the heme biosynthesis pathway for production of valuable chemicals.
文章类型Article
资助机构National key Research and Development Plan of China [2016YFE0106700]; National Natural Science Foundation of China [21373271, 21673294]; Key Research Project of Shandong Province [2017GGX40114]; Open Fund of Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China [OF2015N009]; Fundamental Research Funds for the Central Universities
收录类别SCI
语种英语
关键词[WOS]5-AMINOLEVULINIC ACID; BILE-PIGMENTS; ALLOPHYCOCYANIN; TETRAPYRROLES; CHROMOPHORE; DISORDERS; SPIRULINA; EFFICIENT; GLUCOSE; PROTEIN
研究领域[WOS]Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; Engineering, Chemical
WOS记录号WOS:000442004200004
引用统计被引频次：6[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24506
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.China Univ Petr East China, Ctr Bioengn & Biotechnol, Qingdao 266580, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Ge, Baosheng,Chen, Yao,Yu, Qian,et al. Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli[J]. PROCESS BIOCHEMISTRY,2018,71:23-30.
APAGe, Baosheng,Chen, Yao,Yu, Qian,Lin, Xiaojun,Li, Jiqiang,&Qin, Song.(2018).Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli.PROCESS BIOCHEMISTRY,71,23-30.
MLAGe, Baosheng,et al."Regulation of the heme biosynthetic pathway for combinational biosynthesis of phycocyanobilin in Escherichia coli".PROCESS BIOCHEMISTRY 71(2018):23-30.
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