Sun Yanyu; Wang Hui
; Li Junde; Wang Bin; Qi Cancan; Hu Xiaoke发表期刊JOURNAL OF OCEANOLOGY AND LIMNOLOGY
ISSN2096-5508
2018-07
卷号36期号:4页码:1294-1303
关键词bioremediationnutrient-enhancedbacterial communitiesn-alkane
研究领域Marine & Freshwater Biology; Oceanography
DOI10.1007/s00343-018-6310-y
产权排序[Sun Yanyu; Wang Hui; Li Junde; Wang Bin; Qi Cancan; Hu Xiaoke] Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China; [Sun Yanyu; Li Junde; Wang Bin; Qi Cancan] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oil-contaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.
文章类型Article
资助机构Chinese Academy of Sciences; Chinese Academy of Sciences [KZZD-EW-14]; Science and Technology Program of Shandong Province [2013GHY11534]; National Natural Science Foundation of China [41376138, 41576165]
收录类别SCI
语种英语
关键词[WOS]CRUDE-OIL; SP-NOV.; HYDROCARBON DEGRADATION; CONTAMINATED SOIL; GEN. NOV.; DEGRADING BACTERIUM; MARINE BACTERIUM; SP. NOV.; BIOREMEDIATION; MICROORGANISMS
研究领域[WOS]Limnology; Oceanography
WOS记录号WOS:000441231100024
引用统计被引频次：5[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24502
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Costal Zone Res, Key Lab Coastal Biol & Bioresource Utilizat, Yantai 264003, Peoples R China;
2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Sun Yanyu,Wang Hui,Li Junde,et al. Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities[J]. JOURNAL OF OCEANOLOGY AND LIMNOLOGY,2018,36(4):1294-1303.
APASun Yanyu,Wang Hui,Li Junde,Wang Bin,Qi Cancan,&Hu Xiaoke.(2018).Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities.JOURNAL OF OCEANOLOGY AND LIMNOLOGY,36(4),1294-1303.
MLASun Yanyu,et al."Nutrient-enhanced n-alkanes biodegradation and succession of bacterial communities".JOURNAL OF OCEANOLOGY AND LIMNOLOGY 36.4(2018):1294-1303.
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