Wang, Dan; Yang, Dinglong
; Wang, Qing; Zhao, Ye; Li, Chenghua; Wei, Qianyu; Han, Yijing; Zhao, Jianmin发表期刊FISH & SHELLFISH IMMUNOLOGY
ISSN1050-4648
2018-07
卷号78页码:158-168
关键词Manila clamMacrophage migration inhibitory factorImmune responseEnzymatic activity
研究领域Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
DOI10.1016/j.fsi.2018.04.026
产权排序[Wang, Dan; Li, Chenghua; Wei, Qianyu] Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China; [Yang, Dinglong; Wang, Qing; Han, Yijing; Zhao, Jianmin] Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China; [Zhao, Ye] Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China; [Yang, Dinglong; Wang, Qing; Han, Yijing; Zhao, Jianmin] Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China; [Han, Yijing] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
作者部门中国科学院牟平海岸带环境综合试验站
英文摘要Macrophage migration inhibitory factor (MIF) is an evolutionarily ancient cytokine-like factor and plays a critical role in both innate and adaptive immunity. In the present study, two MIFs (designed as RpMIF-1 and RpMIF-2, respectively) were identified and characterized from the clam Ruditapes philippinarum by rapid amplification of cDNA ends (RACE) approaches. The full-length cDNA of RpMIF-1 and RpMFI-2 consisted of 531 and 722 nucleotides, encoding a polypeptide of 113 and 114 amino acid residues, respectively. Multiple alignments and phylogenetic analysis revealed that both RpMIF-1 and RpMIF-2 belonged to the MIF family. The conserved catalytic-site Pro(2) for tautomerase activity was identified in the deduced amino acid sequences of RpMIFs. Both RpMIF-1 and RpMIF-2 transcripts were constitutively expressed in examined tissues of R. philippinarum with dominant expression in hepatopancreas, gills and hemocytes. Immunolocalization analysis showed that RpMIF-1 and RpMIF-2 proteins were expressed in examined tissues with the exception of adductor muscle and foot. After Vibrio anguillarum and Micrococcus luteus challenge, the mRNA expression of RpMIFs was significantly modulated in hemocytes, gills and hepatopancreas. Recombinant RpMIF-1 and RpMIF-2 proteins possessed significant tautomerase activity and oxidoreductase activity, indicating that these two proteins was perhaps involved in inflammatory responses. In summary, our results suggested that RpMIF-1 and RpMIF-2 played an important role in the innate immunity of R. philippinarum.
文章类型Article
资助机构Strategic Priority Research Program of the Chinese Academy of Sciences [XDA11020702]; National Natural Science Foundation of China [41576122]; Youth Innovation Promotion Association CAS [2016196]; K.C. Wong Magna Fund at Ningbo University
收录类别SCI
语种英语
关键词[WOS]SCALLOP CHLAMYS-FARRERI; INNATE IMMUNE-RESPONSES; TOLL-LIKE RECEPTOR-4; MARINE-INVERTEBRATES; REGULATORY ROLE; DANIO-RERIO; CYTOKINE; GENE; EXPRESSION; INVOLVEMENT
研究领域[WOS]Fisheries; Immunology; Marine & Freshwater Biology; Veterinary Sciences
WOS记录号WOS:000437385000019
引用统计被引频次：4[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24444
专题支撑部门_中国科学院牟平海岸带环境综合试验站
海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
海岸带生物学与生物资源利用重点实验室_海岸带生物资源高效利用研究与发展中心
作者单位1.Ningbo Univ, Ningbo 315211, Zhejiang, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res YIC, Muping Coastal Environm Res Stn, Yantai 264003, Peoples R China;
3.Yantai Univ, Ocean Sch, Yantai 264005, Peoples R China;
4.Chinese Acad Sci, Res & Dev Ctr Efficient Utilizat Coastal Bioresou, Yantai Inst Coastal Zone Res, Yantai 264003, Shandong, Peoples R China;
5.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714Wang, Dan,Yang, Dinglong,Wang, Qing,et al. Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities[J]. FISH & SHELLFISH IMMUNOLOGY,2018,78:158-168.
APAWang, Dan.,Yang, Dinglong.,Wang, Qing.,Zhao, Ye.,Li, Chenghua.,...&Zhao, Jianmin.(2018).Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities.FISH & SHELLFISH IMMUNOLOGY,78,158-168.
MLAWang, Dan,et al."Two macrophage migration inhibitory factors (MIFs) from the clam Ruditapes philippinarum: Molecular characterization, localization and enzymatic activities".FISH & SHELLFISH IMMUNOLOGY 78(2018):158-168.
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