Zheng, Shiling; Liu, Fanghua
; Li, Meng; Xiao, Leilei; Wang, Oumei发表期刊SCIENCE CHINA-LIFE SCIENCES
ISSN1674-7305
2018-07
卷号61期号:7页码:787-798
关键词comparative transcriptomicsGeobacterdirect interspecies electron transfer (DIET)activated carbonmagnetite
研究领域Life Sciences & Biomedicine - Other Topics
DOI10.1007/s11427-017-9177-1
产权排序[Zheng, Shiling; Liu, Fanghua; Xiao, Leilei] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China; [Li, Meng] Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China; [Wang, Oumei] Binzhou Med Univ, Yantai 264003, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Both activated carbon and magnetite have been reported to promote the syntrophic growth of Geobacter metallireducens and Geobacter sulfurreducens co-cultures, the first model to show direct interspecies electron transfer (DIET); however, differential transcriptomics of the promotion on co-cultures with these two conductive materials are unknown. Here, the comparative transcriptomic analysis of G. metallireducens and G. sulfurreducens co-cultures with granular activated carbon (GAC) and magnetite was reported. More than 2.6-fold reduced transcript abundances were determined for the uptake hydrogenase genes of G. sulfurreducens as well as other hydrogenases in those co-cultures to which conductive materials had been added. This is consistent with electron transfer in G. metallireducens-G. sulfurreducens co-cultures as evinced by direct interspecies electron transfer (DIET). Transcript abundance for the structural component of electrically conductive pili (e-pili), PilA, was 2.2-fold higher in G. metallireducens, and, in contrast, was 14.9-fold lower in G. sulfurreducens in co-cultures with GAC than in Geobacters co-cultures without GAC. However, it was 9.3-fold higher in G. sulfurreducens in co-cultures with magnetite than in Geobacters co-cultures. Mutation results showed that GAC can be substituted for the e-pili of both strains but magnetite can only compensate for that of G. sulfurreducens, indicating that the e-pili is a more important electron acceptor for the electron donor strain of G. metallireducens than for G. sulfurreducens. Transcript abundance for G. metallireducens c-type cytochrome gene GMET_RS14535, a homologue to c-type cytochrome gene omcE of G. sulfurreducens was 9.8-fold lower in co-cultures with GAC addition, while that for OmcS of G. sulfurreducens was 25.1-fold higher in co-cultures with magnetite, than in that without magnetite. Gene deletion studies showed that neither GAC nor magnetite can completely substitute the cytochrome (OmcE homologous) of G. metallireducens but compensate for the cytochrome (OmcS) of G. sulfurreducens. Moreover, some genes associated with central metabolism were up-regulated in the presence of both GAC and magnetite; however, tricarboxylic acid cycle gene transcripts in G. sulfurreducens were not highly-expressed in each of these amended co-cultures, suggesting that there was considerable redundancy in the pathways utilised by G. sulfurreducens for electron transfer to reduce fumarate with the amendment of GAC or magnetite. These results support the DIET model of G. metallireducens and G. sulfurreducens and suggest that e-pili and cytochromes of the electron donor strain are more important than that of the electron acceptor strain, indicating that comparative transcriptomics may be a promising route by which to reveal different responses of electron donor and acceptor during DIET in co-cultures.
文章类型Article
资助机构Major Research plan of the National Natural Science Foundation of China [91751112]; General Programme of the National Natural Science Foundation of China [41371257, 41573071]; Shandong Natural Science Fund for Distinguished Young Scholars [JQ201608]; Young Taishan Scholars Programme of Shandong Province [tsqn20161054]; Key Research Project for Frontier Science of the Chinese Academy of Sciences [QYZDJ-SSW-DQC015]
收录类别SCI
语种英语
关键词[WOS]C-TYPE CYTOCHROME; FE(III) OXIDE; MICROBIAL NANOWIRES; GENETIC SYSTEM; SULFURREDUCENS; REDUCTION; METALLIREDUCENS; HYDROGEN; ALIGNMENT; MINERALS
研究领域[WOS]Biology
WOS记录号WOS:000438465700007
引用统计被引频次：10[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24441
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai 264003, Peoples R China;
2.Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China;
3.Binzhou Med Univ, Yantai 264003, Peoples R China
推荐引用方式
GB/T 7714Zheng, Shiling,Liu, Fanghua,Li, Meng,et al. Comparative transcriptomic insights into the mechanisms of electron transfer in Geobacter co-cultures with activated carbon and magnetite[J]. SCIENCE CHINA-LIFE SCIENCES,2018,61(7):787-798.
APAZheng, Shiling,Liu, Fanghua,Li, Meng,Xiao, Leilei,&Wang, Oumei.(2018).Comparative transcriptomic insights into the mechanisms of electron transfer in Geobacter co-cultures with activated carbon and magnetite.SCIENCE CHINA-LIFE SCIENCES,61(7),787-798.
MLAZheng, Shiling,et al."Comparative transcriptomic insights into the mechanisms of electron transfer in Geobacter co-cultures with activated carbon and magnetite".SCIENCE CHINA-LIFE SCIENCES 61.7(2018):787-798.
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