Wang, Oumei; Zheng, Shiling; Wang, Bingchen; Wang, Wenjing; Liu, Fanghua
发表期刊PEERJ
ISSN2167-8359
2018-03-21
卷号6页码:e4541
关键词Geobacter metallireducensMethanosarcina barkeriElectrically conductive pili (e-pili)Direct interspecies electron transfer (DIET)MagnetiteFerrous ironEthanol metabolismStimulationCo-culturesMethane
研究领域Multidisciplinary Sciences
DOI10.7717/peerj.4541
产权排序[Wang, Oumei] Binzhou Med Univ, Yantai, Shandong, Peoples R China; [Zheng, Shiling; Wang, Bingchen; Wang, Wenjing; Liu, Fanghua] Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China; [Wang, Bingchen] Univ Chinese Acad Sci, Beijing, Peoples R China
作者部门海岸带生物学与生物资源保护实验室
英文摘要Background: Magnetite-mediated direct interspecies electron transfer (DIET) between Geobacter and Methanosarcina species is increasingly being invoked to explain magnetite stimulation of methane production in anaerobic soils and sediments. Although magnetite-mediated DIET has been documented in defined co-cultures reducing fumarate or nitrate as the electron acceptor, the effects of magnetite have only been inferred in methanogenic systems. Methods: Concentrations of methane and organic acid were analysed with a gas chromatograph and high-performance liquid chromatography, respectively. The concentration of HCl-extractable Fe(II) was determined by the ferrozine method. The association of the defined co-cultures of G. metallireducens and M. barkeri with magnetite was observed with transmission electron micrographs. Results: Magnetite stimulated ethanol metabolism and methane production in defined co-cultures of G. metallireducens and M. barkeri; however, magnetite did not promote methane production in co-cultures initiated with a culture of G. metallireducens that could not produce electrically conductive pili (e-pili), unlike the conductive carbon materials that facilitate DIET in the absence of e-pili. Transmission electron microscopy revealed that G. metallireducens and M. barkeri were closely associated when magnetite was present, as previously observed in G. metallireducens/G. sulfurreducens co-cultures. These results show that magnetite can promote DIET between Geobacter and Methanosarcina species, but not as a substitute for e-pili, and probably functions to facilitate electron transfer from the e-pili to Methanosarcina. Conclusion: In summary, the e-pili are necessary for the stimulation of not only G. metallireducens/G. sulfurreducens, but also methanogenic G. metallireducens/M. barkeri co-cultures with magnetite.
文章类型Article
资助机构Major Research Plan of the National Natural Science Foundation of China [91751112]; General Programme of the National Natural Science Foundation of China [41573071, 41371257]; Key Research Project of Frontier Science of the Chinese Academy of Sciences [QYZDJ-SSW-DQC015]; Natural Science Foundation of Shandong Province [ZR2016DQ12]; Young Taishan Scholars Programme [tsqn20161054]
收录类别SCI
语种英语
关键词[WOS]INTERSPECIES ELECTRON-TRANSFER; C-TYPE CYTOCHROME; GEOBACTER-METALLIREDUCENS; PADDY SOIL; OXIDE REDUCTION; FE(III) OXIDE; SP-NOV; EXCHANGE; METHANE; SULFURREDUCENS
研究领域[WOS]Science & Technology - Other Topics
WOS记录号WOS:000428693200003
引用统计被引频次：17[WOS][WOS记录][WOS相关记录]
文献类型期刊论文
条目标识符http://ir.yic.ac.cnhttp://ir.yic.ac.cn/handle/133337/24383
专题海岸带生物学与生物资源利用重点实验室_海岸带生物学与生物资源保护实验室
作者单位1.Binzhou Med Univ, Yantai, Shandong, Peoples R China;
2.Chinese Acad Sci, Yantai Inst Coastal Zone Res, Key Lab Coastal Biol & Biol Resources Utilizat, Yantai, Shandong, Peoples R China;
3.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714Wang, Oumei,Zheng, Shiling,Wang, Bingchen,et al. Necessity of electrically conductive pili for methanogenesis with magnetite stimulation[J]. PEERJ,2018,6:e4541.
APAWang, Oumei,Zheng, Shiling,Wang, Bingchen,Wang, Wenjing,&Liu, Fanghua.(2018).Necessity of electrically conductive pili for methanogenesis with magnetite stimulation.PEERJ,6,e4541.
MLAWang, Oumei,et al."Necessity of electrically conductive pili for methanogenesis with magnetite stimulation".PEERJ 6(2018):e4541.
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