学历
博士

职称
教授

所属部门
生物化学与分子生物学系

招生专业
生物化学与分子生物学（硕士、博士）

联系方式
电话: E-mail: xyunwang@sdau.edu.cn

个人简介

王晓云，博士，教授，博士生导师。 2002-2003年清华大学访问****，2008-2010年哈佛大学访问****，2011年获得高等学校教学名师称号。主持教育部双语建设课程，是国家级教学团队的骨干成员。主持国家自然基金等项目，作为第一作者或通讯作者在“Antioxidant and Redox signaling” “Scientific Reports” “Molecular Genetics and Genomics”“International Journal of Biochemistry and Cell Biology” “Frontier in Plant Science”“BMC Plant Biol”等学术期刊发表学术论文60多篇。

教学工作

承担本科生《生物化学》、《分子生物学》、《基础生物化学试验》；硕士研究生《生物化学》等课程的教学工作，主持教育部生物化学双语建设课程、山东省首届精品课程和山东省重点建设课程，是国家级教学团队的骨干成员。

研究方向

1.生物化学与分子生物学
2.植物抗逆分子生物学

科研项目

1.国家自然科学基金“MdRAf5基因介导的苹果砧木干旱抗性分子调控网络解析”
2.农业部公益性行业科研专项“我国重要野生果树资源的收集、评价与优异种质创新利用技术研究与示范” （主要参加人）
3.国家自然科学基金 “利用点突变技术研究二硫键对植酸酶结构、催化活性及热稳定性的作用”
4.国家自然科学基金“通过引物二硫键、增加糖基化位点等方式提高植酸热稳定性的研究”
5.高等学校博士点专项基金：“糖基化及二硫键对植酸酶稳定性的研究”
6.山东省中青年科学家科研奖励基金“叶片衰老关键酶分离纯化及调控衰老物质的研制与开发”

发表论文

1.Xu, J., Xing, S., Sun, Q., Zhan, C., Liu, X., Zhang, S. * & Wang, X.Y*. The expression of a tubby-like protein from Malus domestica (MdTLP7) enhances abiotic stress tolerance in Arabidopsis, BMC Plant Biol. 2019, 19, 60.
2.Liu, X., Li, R., Dai, Y., Yuan, L., Sun, Q., Zhang, S. * & Wang, X.Y. * A B-box zinc finger protein, MdBBX10, enhanced salt and drought stresses tolerance in Arabidopsis, Plant Mol Biol. 2019, doi.org/10.1007/s11103-019-00828-8
3.Li, M., Wei, G., Shi, W., Sun, Z., Li, H., Wang, X.Y. *, & Gao, Z. * Distinct distribution patterns of ammonia-oxidizing archaea and bacteria in sediment and water column of the Yellow River estuary. Sci Rep, 2018, 8: 1584-1594.
4.Liu, X., Li, R., Dai, Y., Chen, X., & Wang, X.Y.* Genome-wide identification and expression analysis of the B-box gene family in the Apple (Malus domestica Borkh.) genome. Mol Genet Genomics. 2018, 293:303-315
5.Wu, Q. Y., Zhu, Y. Y., Wei, F., Tong, Y. X., Cao, J., Zhou, P., Li, Z. Y., Zeng, L. Y., Li, F., Wang, X. Y. *, & Xu, K. L. Mutation of the conserved G66 residue in GS region decreased structural stability and activity of arginine kinase. Int J Biol Macromol, 2018, 111: 247-254.
6.Zhang, Z., Liu, X., Li, R., Yuan, L., Dai, Y., & Wang, X.Y.* Identification and functional analysis of a protein disulfide isomerase (AtPDI1) in Arabidopsis thaliana. Front Plant Sci, 2018, 9: 913-923.
7.Zhang, Z., Yuan, L., Liu, X., Chen, X., & Wang, X.Y*. Evolution analysis of Dof transcription factor family and their expression in response to multiple abiotic stresses in Malus domestica. Gene, 2018, 639: 137-148.
8.Wu, Q. Y., Wei, F., Zhu, Y. Y., Tong, Y. X., Cao, J., Zhou, P., Li, Z. Y., Zeng, L. Y., Li, F., Wang, X. Y. *, & Xu, K. L. Roles of amino acid residues H66 and D326 in the creatine kinase activity and structural stability. Int J Biol Macromol. 2017, 107: 512-520
9.Xu, J., Xing, S., Cui, H., Chen, X., & Wang, X.Y*. Genome-wide identification and characterization of the apple (Malus domestica) HECT ubiquitin-protein ligase family and expression analysis of their responsiveness to abiotic stresses. Mol Genet Genomics, 2016, 291: 635-646.
10.Xu, J. N., Xing, S. S., Zhang, Z. R., Chen, X. S., & Wang, X. Y*. Genome-Wide identification and expression analysis of the tubby-like protein family in the Malus domestica genome. Front Plant Sci, 2016, 7: 1693.
11.Yu, Z. B., Yang, X. J., Du, J. J., Wan, C. M., Xu, J. N., Wang, W. J., Feng, Y. G., & Wang, X. Y*. A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress. Physiol Plant, 2016, 156(3): 311-322.
12.Mao, Y., Hopkins, D. L., Zhang, Y., Li, P., Zhu, L., Dong, P., Liang, R., Dai, J., Wang, X.Y. *, & Luo, X. Beef quality with different intramuscular fat content and proteomic analysis using isobaric tag for relative and absolute quantitation of differentially expressed proteins. Meat Sci, 2016, 118: 96-102.
13.Yang X. J., Cui H. R., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Key amino acids of Arabidopsis VKOR in the activity of phylloquinone reduction and disulfide bond formation. Protein Pept Lett. 2015, 22:81-86.
14.Xu J. N., Xing S. S., Cui H. R., Chen X. S., and Wang X. Y.*, Genome-wide identification and characterization of the apple (Malus domestica) HECT ubiquitin-protein ligase family and expression analysis of their responsiveness to abiotic stresses. Mol Genet Genomics. 2015. DOI:10.1007/s00438-015-1129-0.
15.Lu Y., Peng J. J., Yu Z. B., Du J. J., Xu J. N., and Wang X. Y.*, Thylakoid membrane oxidoreductase LTO1/AtVKOR is involved in ABA-mediated response to osmotic stress in Arabidopsis. Physiol Plant. 2015, 154:28-38.
16.Lu Y., Du J. J., Yu Z. B., Peng J. J., Xu J. N., and Wang X. Y.*, Identification of potential targets for thylakoid oxidoreductase AtVKOR/LTO1 in chloroplasts. Protein Pept Lett. 2015, 22:219-225.
17.Li F., Li W., Liu Y., Tong Y. X., Zhou P., Wang L., Chen C., Zeng L. Y., Wu Q. Y., Wang X. Y. *, and Xu K. L., Effects of the I682F mutation on JAK2's activity, structure and stability. Int J Biol Macromol. 2015, 79:118-125.
18.Du J. J., Zhan C. Y., Lu Y., Cui H. R., and Wang X. Y.*, The conservative cysteines in transmembrane domain of AtVKOR/LTO1 are critical for photosynthetic growth and photosystem II activity in Arabidopsis. Frontiers in Plant Science. 2015, 6:238.
19.Cui H. R., Zhang Z. R., Lv W., Xu J. N., and Wang X. Y.*, Genome-wide characterization and analysis of F-box protein-encoding genes in the Malus domestica genome. Molecular Genetics and Genomics. 2015, 290:1-12.
20.Jing X. L., Xu J., Fan M. N., Ma L., Huang X., Wang X. Y., Sun S. H., Zhu C. X., and Liu H. M., Mutation analysis of the RNA silencing suppressor NS1 encoded by avian influenza virus H9N2. Journal of General Virology. 2015, 96:1613-1618
21.Zhan C. Y., Xu J. N., Li D., Liu B. B., Feng Y. G., and Wang X. Y. *, Overexpression of AtCBF1 in Sinningia speciosa Enhances Chilling Stress Tolerance. Journal of Botanical Sciences. 2015, S1：26-33.
22.Wu Q. Y., Guo H. Y., Geng H. L., Ru B. M., Cao J., Chen C., Zeng L. Y., Wang X. Y., Li F., and Xu K. L., T273 plays an important role in the activity and structural stability of arginine kinase. Int J Biol Macromol. 2014, 63:21-28.
23.Wang P., Li X. Z., Cui H. R., Feng Y. G., and Wang X. Y*., Identification and functional analysis of a novel parvulin-type peptidyl-prolyl isomerase from Gossypium hirsutum. Plant Physiol Biochem. 2014, 76C:58-66.
24.Wan C. M., Yang X. J., Du J. J., Lu Y., Yu Z. B., Feng Y. G., and Wang X. Y*., Identification and characterization of SlVKOR, a disulfide bond formation protein from Solanum lycopersicum, and bioinformatic analysis of plant VKORs. Biochemistry (Mosc). 2014, 79:440-449.
25.Geng H. L., Bian M. R., Liu Y., Cao J., Chen C., Wang Z. Y., Li Z. Y., Zeng L. Y., Wang X. Y. *, Wu Q. Y., and Xu K. L., The D14 and R138 ion pair is involved in dimeric arginine kinase activity, structural stability and folding. Int J Biol Macromol. 2014, 66:302-310.
26.Du F., Xu J. N., Zhan C. Y., Yu Z. B., and Wang X. Y.*, An obesity-like gene MdTLP7 from apple (Malus domestica) enhances abiotic stress tolerance. Biochemical and biophysical research communications. 2014, 445:394-397.
27.Du F., Xu J. N., Li D., and Wang X. Y.*, The identification of novel and differentially expressed apple-tree genes under low-temperature stress using high-throughput Illumina sequencing. Mol Biol Rep. 2014, 42:569-580.
28.Yu Z. B., Lu Y., Du J. J., Peng J. J., and Wang X. Y.*, The chloroplast protein LTO1/AtVKOR is involved in the xanthophyll cycle and the acceleration of D1 protein degradation. J Photochem Photobiol B. 2013, 130C:68-75.
29.Lu Y., Wang H. R., Li H., Cui H. R., Feng Y. G., and Wang X. Y*., A chloroplast membrane protein LTO1/AtVKOR involving in redox regulation and ROS homeostasis. Plant Cell Rep. 2013, 32:1427-1440.
30.Li F., Wu Q. Y., and Wang X. Y., The amino acid residue L113 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2013, 52:198-205.
31.Wu Q. Y., Li F., Wang X. Y. *, and Xu K. L., Evidence that the amino acid residue Ile121 is involved in arginine kinase activity and structural stability. Int J Biol Macromol. 2012, 51:369-377.
32.Wu Q. Y., Li F., Guo H. Y., Cao J., Chen C., Chen W., Zhao K., Zeng L. Y., Han Z. X., Li Z. Y., Wang X. Y. *, and Xu K. L., Amino acid residue E543 in JAK2 C618R is a potential therapeutic target for myeloproliferative disorders caused by JAK2 C618R mutation. Arch Biochem Biophys. 2012, 528:57-66.
33.Wu Q. Y., Li F., Wang X. Y. *, and Chen Z. J., Impact of inter-subunit interactions on the dimeric arginine kinase activity and structural stability. Arch Biochem Biophys. 2011, 512:61-68.
34.Wu Q. Y., Jin K. Z., Li F., Hu Z. Q., and Wang X. Y.*, Impact of intra-subunit interactions on the dimeric arginine kinase activity and structural stability. Int J Biol Macromol. 2011, 49:822-831.
35.Wang X. Y., Dutton R. J., Beckwith J., and Boyd D., Membrane topology and mutational analysis of Mycobacterium tuberculosis VKOR, a protein involved in disulfide bond formation and a homologue of human vitamin K epoxide reductase. Antioxid Redox Signal. 2011, 14:1413-1420.
36.Wang H. R., Zhu W. J., and Wang X. Y.*, Mechanism of inhibition of arginine kinase by flavonoids consistent with thermodynamics of docking simulation. Int J Biol Macromol. 2011, 49:985-991.
37.Jing X. L., Fan M. N., Jia G., Liu L. W., Ma L., Zheng C. C., Zhu X. P., Liu H. M., and Wang X. Y.*, A Multifunctional Protein Encoded by Turkey Herpesvirus Suppresses RNA Silencing in Nicotiana benthamiana. J Virol. 2011, 85:12792-12803.
38.Feng W. K., Wang L., Lu Y., and Wang X. Y.*, A protein oxidase catalysing disulfide bond formation is localized to the chloroplast thylakoids. FEBS J. 2011, 278:3419-3430.
39.Li F., Wu Q. Y., Sun Y. L., Ma N. N., Wang X. Y., and Meng Q. W., Evidence that the amino acid residue Cys117 of chloroplastic monodehydroascorbate reductase is involved in its activity and structural stability. Int J Biol Macromol. 2010, 46:350-355.
40.Wu X. Q., Zhu W. J., Lu Z. R., Xia Y., Yang J. M., Zou F., and Wang X. Y.*, The effect of rutin on arginine kinase: inhibition kinetics and thermodynamics merging with docking simulation. Int J Biol Macromol. 2009, 44:149-155.
41.Wu X. Q., Xu H., Yue H., Liu K. Q., and Wang X. Y.*, Inhibition kinetics and the aggregation of alpha-glucosidase by different denaturants. Protein J. 2009, 28:448-456.
42.Wu Q. Y., Li F., and Wang X. Y.*, Val65 plays an important role in the substrate synergism, structural stability and activity of arginine kinase. Int J Biol Macromol. 2009, 45:393-398.
43.Shi X. W., Sun M. L., Zhou B., and Wang X. Y.*, Identification, characterization, and overexpression of a phytase with potential industrial interest. Can J Microbiol. 2009, 55:599-604.
44.Zhou J. P., Yang Z. B., Yang W. R., Wang X. Y., Jiang S. Z., and Zhang G. G., Effects of a New Recombinant Phytase on the Performance and Mineral Utilization of Broilers Fed Phosphorus-Deficient Diets. The Journal of Applied Poultry Research. 2008, 17:331.
45.Wu Q. Y., Li F., and Wang X. Y.*, Evidence that amino-acid residues are responsible for substrate synergism of locust arginine kinase. Insect Biochem Mol Biol. 2008, 38:59-65.
46.Wu Q. Y., Li F., and Wang X. Y.*, Evidence that the amino acid residue P272 of arginine kinase is involved in its activity, structure and stability. Int J Biol Macromol. 2008, 43:367-372.
47.Zhu W. J., Li M., and Wang X. Y.*, Chemical modification studies on arginine kinase: essential cysteine and arginine residues at the active site. Int J Biol Macromol. 2007, 41:564-571.
48.Zhao D. M., Wang M., Mu X. J., Sun M. L., and Wang X. Y.*, Screening, cloning and overexpression of Aspergillus niger phytase (phyA) in Pichia pastoris with favourable characteristics. Lett Appl Microbiol. 2007, 45:522-528.
49.Xia Y., Park Y. D., Mu H., Zhou H. M., Wang X. Y.*, and Meng F. G., The protective effects of osmolytes on arginine kinase unfolding and aggregation. Int J Biol Macromol. 2007, 40:437-443.
50.Wu Q. Y., Li F., Zhu W. J., and Wang X. Y.*, Cloning, expression, purification, and characterization of arginine kinase from Locusta migratoria manilensis. Comp Biochem Physiol B Biochem Mol Biol. 2007, 148:355-362.