喻国洪¹, 王扬才², 金中文²
1.宁波大学海洋学院, 浙江 宁波 315832;2.宁波市海洋与渔业研究院, 浙江 宁波 315408

摘要:
为比较第一次和第二次卵巢发育成熟拟穴青蟹卵巢和肝胰腺营养成分的差异。作者选取一次卵巢发育成熟和二次卵巢发育成熟的拟穴青蟹（Scylla paramamosain）各5只，对其卵巢和肝胰腺的常规营养成分、氨基酸和脂肪酸含量进行检测和分析，比较两次卵巢发育青蟹卵巢和肝胰腺主要营养成分的异同，并对其营养价值进行的分析和评估。结果显示：卵巢二次发育青蟹性腺指数（GSI）为9.29±1.81，显著低于一次卵巢发育青蟹的15.46±1.81。C16：0（棕榈酸）、C18：0（硬脂酸）、C16：1（ω–7）（棕榈油酸）、C18：1（ω–9）（油酸）、C20：5（ω–3）（EPA）和C22：6（ω–3）（DHA）为卵巢和肝胰腺主要脂肪酸组成，卵巢中饱和脂肪酸（SFA）、多不饱和脂肪酸（MUFA）、单不饱和脂肪酸（PUFA）和总脂肪酸（TFA）在二次卵巢发育青蟹和一次卵巢发育青蟹均无显著差异（P>0.05）。而两次卵巢发育成熟青蟹肝胰腺中TFA、SFA、MUFA以及PUFA均显著差异（P<0.05），主要的能量型脂肪酸C16：0、C18：0、C16：1、C18：1，DHA和EPA二次卵巢发育青蟹显著低于一次卵巢发育青蟹（P<0.05）。青蟹卵巢和肝胰腺中氨基酸组成相同，谷氨酸含量最高，其次为精氨酸，半胱氨酸含量最低。无论卵巢还是肝胰腺，卵巢二次发育青蟹的必需氨基酸（EAA）、非必需氨基酸（NEAA）、总氨基酸（TAA）含量与卵巢一次发育青蟹无显著差异（P>0.05），青蟹卵巢中总氨基酸含量明显高于肝胰腺。二次卵巢发育的青蟹生殖力低于一次卵巢发育青蟹，尽管卵巢二次发育时间短，但青蟹卵巢中脂肪酸和氨基酸的含量和组成与一次卵巢发育青蟹相当，其生殖性能接近一次卵巢发育青蟹。而肝胰腺由于初次繁殖中能量消耗及二次卵巢发育过程对脂肪酸需求，而导致脂肪酸含量明显低于一次卵巢发育青蟹。
关键词:拟穴青蟹(Scylla paramamosain)卵巢发育脂肪酸氨基酸
DOI：10.11759/hykx20200908003
分类号:S963
基金项目:浙江省宁波市科技局科技创新2025重大专项（2019B10010）

Comparative analysis of nutrient composition of the ovaries and hepatopancreas in the mud crab Scylla paramamosain at two stages of ovary maturation
YU Guo-hong¹, WANG Yang-cai², JIN Zhong-wen²
1.School of Marine Sciences, Ningbo University, Zhejiang Province, Ningbo 315832, China;2.Ningbo Ocean and Fisheries Research Institute of Zhejiang Province, Ningbo 315408, China
Abstract:
To compare the nutrient composition of the ovaries and hepatopancreas in the primary and secondary fully-developed ovaries of mud crab, Scylla paramamosain. Five mature mud crabs with primary and secondary ovary development were selected for detection and analysis of the conventional nutritional, amino acid, and fatty acid contents of the ovaries and hepatopancreas and to compare the development of the mud crab ovaries and hepatopancreas. The similarities and differences in the main nutrients were analyzed and their nutritional value evaluated. The results showed that the gonadal index (GSI) of mud crabs with secondary ovarian development was 9.29±1.81, which was significantly lower than that of mud crabs with primary ovarian development 15.46±1.81. C16:0 (palmitic acid), C18:0 (stearic acid), C16:1(ω-7) (palmitoleic acid), C18:1(ω-9) (oleic acid), C20:5(ω-3) (EPA), and C22:6(ω-3) (DHA) were the main fatty acids in the ovary and hepatopancreas. With respect to saturated, monounsaturated, polyunsaturated, and total fatty acids in the ovary, there was no significant difference between primary and secondary ovary development in mud crabs (P>0.05). Although total, saturated, monounsaturated, and polyunsaturated fatty acids in the hepatopancreas were significantly different (P<0.05), the main energy-yielding fatty acids C16:0, C18:0, C16:1, C18:1, DHA, and EPA were significantly lower in mud crabs with secondary vs. primary ovary development (P<0.05). Overall, the results showed that the fertility of mud crabs with secondary vs. primary ovary development. Although secondary ovary development time was short, the content and composition of fatty acids in the ovaries of mud crabs were equivalent in both developmental stages, and their reproductive performances were comparable. The fatty acid content of the hepatopancreas was significantly lower than the primary ovary development of mud crab due to energy consumption during primary reproduction and the need for fatty acids during secondary ovary development.
Key words:Scylla paramamosainovary developmentfatty acidsamino acids





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