刘建国^1,2,3
1.中国科学院海洋研究所 海洋大科学中心 实验海洋生物学重点实验室, 山东 青岛 266071;2.青岛海洋科学与技术试点国家实验室 海洋生物学与生物技术功能实验室, 山东 青岛 266071;3.红球藻种质培育与虾青素制品开发国家地方联合工程中心, 云南 楚雄 675012

摘要:
经30多年不懈努力与系统创新研发，我国已成功地实现红球藻虾青素资源的规模化生产，开发出新资源食品和功能性生物制品。期间，我们结合微藻产业现状与国情，围绕红球藻资源开发的产业化链条，针对上中下游重要环节的关键性瓶颈问题，开展了基础理论探索、新技术开发、产业化推广与新活性功效发掘等系列工作。借建所70周年所庆成果专刊出版之际，全面回顾总结我们在该藻种质资源、基于细胞周期调控的二步串联培养模式、关键参数优化与控制、光反应器培养设施创制、生物污染危害和防控原理与策略、活性物质开发与新功能挖掘、产业标准与体系建设等方面所取得的进展，同时展望发展趋势，以期促进红球藻以及整个微藻产业的健康可持续发展。
关键词:红球藻虾青素大型光生物反应器敌害生物防治细胞周期及调控
DOI：10.11759/hykx20200314003
分类号:Q-1
基金项目:国家自然科学基金（39500114，39970575，31572639，U1706209，31872588，31702366）；国际科学基金（A/2786-1，A/2786-2）；科技部项目（02EFN216601213，2008AA09Z403，SQ2009AA10XK1485694，2011CB200904，2011CB200901，2018TFE0107200）；中国科学院创新项目（KGCX2-YW-373-3，Y12510101L，Y12509101L，ZSTH-024）

Development history and future prospects of culturing Haematococcus pluvialis for natural astaxanthin
LIU Jian-guo^1,2,3
1.CAS Key Laboratory of Experimental Marine Biology, Center for Ocean Mega-Science, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;2.Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology(Qingdao), Qingdao 266071, China;3.National-Local Joint Engineering Research Center for Haematococcus Pluvialis and Astaxanthin Products, Chuxiong 675012, China
Abstract:
Mass-scale cultivation of unicellular Haematococcus pluvialis for natural astaxanthin as a new source of food and functional byproduct has been successfully realized in China because of research and development in the last 30 years. During this period, our research group in IOCAS studied basic scientific principles, developed theoretical and technological knowledge, and performed industrial application and promotion, as well as discovered new solutions to the bottlenecks at important nodes of upstream, midstream, and downstream processes in the H. pluvialis cultivation and astaxanthin production chain, according to the national economic situation, technique conditions, and development status of the microalgal industry. On the occasion of the publication of this special issue to celebrate the 70th anniversary of IOCAS, we provide a brief review of our main achievements on each aspect of H. pluvialis cultivation to promote the sustainable development of H. pluvialis and the entire microalgal industry. The review summarizes the upstream, midstream, and downstream processes, including germplasm resources, cell-cycle-dependent two-step culture mode, and culture optimization. In addition, other key parameters such as automatic control, establishment of closed facilities and photobioreactor scale-up, biocontamination prevention and progress control, bioactive substance extraction and its biofunctional discovery, industry standards, and system construction have also been summarized. Moreover, new research subjects and development trends are also put forward.
Key words:Haematococcus pluvialisastaxanthinlarge-scale photobioreactorcontamination controlcell cycle and regulation





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