郭丽霞, 宁寰宇, 曹苇, 李慧, 孔亮, 谭成玉
大连海洋大学海洋科技与环境学院, 辽宁 大连116023

摘要:
建立RP-HPLC （反相高效液相色谱法）同时测定翅碱蓬中四种黄酮化合物-芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素的含量。采用Kromasil C18色谱柱（4.6 mm×250 mm，5 μm），检测波长为361 nm，柱温为30℃，流动相为甲醇-0.4%冰乙酸（55：45），流速1.0 mL/min，四种黄酮类化合物得到很好的分离。芸香苷、槲皮素3-β-D-葡萄糖苷、水仙苷和槲皮素浓度均为0.001~0.01 mg/mL范围内呈现良好线性关系，相关系数R²在0.999 0~0.999 6，加标回收率（n=3）范围为82.39%~99.03%。该方法简单、快速、高效，可以用于翅碱蓬中四种黄酮化合物的含量分析。
关键词:翅碱蓬反相高效液相色谱芸香苷槲皮素3-β-D-葡萄糖苷水仙苷槲皮素
DOI：10.11759/hykx20190716003
分类号:O629.9
基金项目:辽宁省自然科学基金重点项目（20170540102），盘锦红海滩湿地退化与生态修复项目（PHL-XZ-2017013002）

Simultaneous determination of four flavonoids in Suaeda Heteroptera by RP-HPLC
GUO Li-xia, NING Huan-yu, CAO Wei, LI Hui, KONG Liang, TAN Cheng-yu
College of Marine Science and Environment, Dalian Ocean University, Dalian 116023, China
Abstract:
In this study, we established a reversed-phase high-performance liquid chromatography (RP-HPLC) method for the simultaneous determination of four flavonoids—rutin, quercetin 3-β-D-glucoside, narcissoside, and quercetin—in Suaeda Heteroptera. The separation was achieved using a Kromasil C18 column (4.6 mm×250 mm, 5 μm) at 30℃, with methanol-0.4% glacial acetic acid (55︰45) as the mobile phase at a flow rate of 1.0 mL.min^-1 and a detection wavelength of 361 nm. The correlation coefficients ranged between 0.999 0 and 0.999 6 with calibration curves in the range of 0.001-0.01 mg·mL^-1 for the four flavones. The average recoveries (n=9) obtained were 82.39%-99.03%. This method is simple, rapid, and efficient, and can be used for the simultaneous determination of rutin, quercetin 3-β-D glucoside, narcissoside, and quercetin in Suaeda Heteroptera samples.
Key words:Suaeda HeteropteraRP-HPLCflavonoidsrutinquercetin 3-β-D-glucosidenarcissosidequercetin





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