宋宗诚¹, 王学颖^2,3,4, 薛蕊^2,3,4, 肖志忠^2,3,4, 马道远^2,3,4, 刘清华^2,3,4, 李军^2,3,4
1.威海圣航水产有限公司, 山东 威海 264200;2.中国科学院海洋研究所 实验海洋生物学重点实验室, 山东 青岛 266071;3.青岛海洋科学与技术国家实验室 海洋生物学与生物技术功能实验室, 山东 青岛 266237;4.中国科学院 海洋大科学研究中心, 山东 青岛 266071

摘要:
为了探明大菱鲆（Scophthalmus maximus）卵子在卵巢腔内老化的原因及特征，采用连续跟踪采卵的方式，通过荧光染色、生理生化指标测定，对不同采卵时间（0，3，6，9，12 h）的卵子活性氧和脂质过氧化情况（氧化产物丙二醛MDA）、抗氧化系统（超氧化物歧化酶SOD，还原型谷胱甘肽GSH）、卵巢液pH值及受精率和孵化率进行对比研究。结果表明，随着卵子在体内老化时间的延长，其活性氧含量及脂质过氧化程度逐渐升高，活性氧含量及过氧化程度与排卵后卵子老化呈正相关；SOD酶活力，GSH酶活力均显著下降，MDA含量显著提高；受精率和孵化率均显著下降。排卵后6 h，孵化率降到2.87%~10.60%，此时的卵质已经极度恶化，不能再继续用于后续的生产中。综上，在卵巢腔中，卵子排放后，随着时间的延长，活性氧含量显著升高，抗氧化能力逐渐下降，受精率和孵化率显著降低，老化逐渐加剧。
关键词:大菱鲆(Scophthalmus maximus)卵子老化活性氧受精率孵化率
DOI：10.11759/hykx20190314002
分类号:S965.3
基金项目:中国博士后科学基金项目（2016M592259）；现代农业产业技术体系专项资金资助项目（CARS-47）

Study on characteristics of post-ovulation aging in ovarian cavity of turbot
SONG Zong-cheng¹, WANG Xue-ying^2,3,4, XUE Rui^2,3,4, XIAO Zhi-zhong^2,3,4, MA Dao-yuan^2,3,4, LIU Qing-hua^2,3,4, LI Jun^2,3,4
1.Weihai Shenghang Aquatic Science and Technology Co., LTD, Weihai 264200, China;2.Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;3.Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266237, China;4.Center for Ocean Mega-Science, Chinese Academy of Sciences, 7 Nanhai Road, Qingdao, 266071, China
Abstract:
Using fluorescence staining, physiological, and biochemical methods, we investigated the characteristics of postovulatory aging in ovarian cavity of turbot. Lipid peroxidation and antioxidant system (superoxide dismutase (SOD), reducing glutathione (GSH), and oxidation product malondialdehyde (MDA)), ovarian fluid pH, and fertilization and hatching rates were detected. Five time points (0, 3, 6, 9, and 12 h) were set for the artificial collection of eggs and fertilization. The results showed that with delayed fertilization of eggs in ovarian cavity, the ROS content and degree of lipid peroxidation gradually increased. ROS and peroxidation were positively correlated with postovulation aging. The concentrations of antioxidant enzyme SOD and GSH decreased, whereas that of MDA significantly increased. Fertilization rate and hatchability significantly declined. At 6 h postovulation, the hatchability dropped to 2.87% to 10.60%, and the eggs cannot be used at this point. In summary, in ovarian cavity, after ovulation, ROS content significantly increases with the extension of time, whereas fertilization rate and hatchability considerably decrease. ROS mainly causes postovulation aging. The present study revealed the basic characteristics of postovulation aging of turbot, determined the optimal time for egg collection, and bears importance in the cultivation of high-quality turbot larva.
Key words:Scophthalmus maximuspostovulatory agingreactive oxygenfertilization ratehatching rate





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